Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics

Hadar Bootz-Maoz; Ariel Simon; Sara Del Mare-Roumani; Yifat Bennet; Einat Toister; Hadar Romano; Danping Zheng; Sivan Amidror; Eran Elinav; Nissan Yissachar

doi:10.1038/s41522-023-00409-0

Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics

Hadar Bootz-Maoz
, Ariel Simon
, Sara Del Mare-Roumani
, Yifat Bennet
, Einat Toister
, Hadar Romano
, Danping Zheng
, Sivan Amidror
, Eran Elinav
, Nissan Yissachar

Weizmann Institute of Science, Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

Abstract

The intestinal epithelial barrier facilitates homeostatic host–microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.

Original language	English
Article number	44
Number of pages	6
Journal	npj Biofilms and Microbiomes
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41522-023-00409-0
State	Published - 3 Jul 2023

ASJC Scopus subject areas

Biotechnology
Microbiology
Applied Microbiology and Biotechnology

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10.1038/s41522-023-00409-0

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title = "Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics",

abstract = "The intestinal epithelial barrier facilitates homeostatic host–microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.",

author = "Hadar Bootz-Maoz and Ariel Simon and \{Del Mare-Roumani\}, Sara and Yifat Bennet and Einat Toister and Hadar Romano and Danping Zheng and Sivan Amidror and Eran Elinav and Nissan Yissachar",

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AU - Bootz-Maoz, Hadar

AU - Simon, Ariel

AU - Del Mare-Roumani, Sara

AU - Bennet, Yifat

AU - Toister, Einat

AU - Romano, Hadar

AU - Zheng, Danping

AU - Amidror, Sivan

AU - Elinav, Eran

AU - Yissachar, Nissan

PY - 2023/7/3

Y1 - 2023/7/3

N2 - The intestinal epithelial barrier facilitates homeostatic host–microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.

AB - The intestinal epithelial barrier facilitates homeostatic host–microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.

UR - https://www.scopus.com/pages/publications/85164000998

U2 - 10.1038/s41522-023-00409-0

DO - 10.1038/s41522-023-00409-0

M3 - مقالة

C2 - 37400593

SN - 2055-5008

VL - 9

JO - npj Biofilms and Microbiomes

JF - npj Biofilms and Microbiomes

IS - 1

M1 - 44

ER -

Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics

Abstract

ASJC Scopus subject areas

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