Ex Vivo Expanded 3D Human Kidney Spheres Engraft Long Term and Repair Chronic Renal Injury in Mice

Orit Harari-Steinberg; Dorit Omer; Yehudit Gnatek; Oren Pleniceanu; Sanja Goldberg; Osnat Cohen-Zontag; Sara Pri-Chen; Nissim Ben Haim; Eli Becker; Roi Ankawa; Yaron Fuchs; Tomer Kalisky; Zohar Dotan; Benjamin Dekel

doi:https://doi.org/10.1016/j.celrep.2019.12.047

Ex Vivo Expanded 3D Human Kidney Spheres Engraft Long Term and Repair Chronic Renal Injury in Mice

Orit Harari-Steinberg, Dorit Omer, Yehudit Gnatek, Oren Pleniceanu, Sanja Goldberg, Osnat Cohen-Zontag, Sara Pri-Chen, Nissim Ben Haim, Eli Becker, Roi Ankawa, Yaron Fuchs, Tomer Kalisky, Zohar Dotan, Benjamin Dekel

Tel Aviv University, Bar-Ilan University, Technion - Israel Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Human kidney cells quickly degenerate in culture, precluding them as a source for cell therapy for chronic kidney disease (CKD). Herein, Harari-Steinberg et al. show that upon culturing as 3D nephrospheres, human kidney cells regain their epithelial phenotype and acquire in vivo tissue-forming and reparative properties in a CKD mouse model.

Original language	English
Pages (from-to)	852-869.e4
Journal	Cell Reports
Volume	30
Issue number	3
DOIs	https://doi.org/10.1016/j.celrep.2019.12.047
State	Published - 21 Jan 2020

Keywords

3D culture
5/6 nephrectomy
antifibrotic effect
autologous treatment
in vivo differentiation
kidney regeneration
kidney stem cells
mouse CKD model
nephrospheres
renal epithelial differentiation

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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https://doi.org/10.1016/j.celrep.2019.12.047

Cite this

Harari-Steinberg, O., Omer, D., Gnatek, Y., Pleniceanu, O., Goldberg, S., Cohen-Zontag, O., Pri-Chen, S., Ben Haim, N., Becker, E., Ankawa, R., Fuchs, Y., Kalisky, T., Dotan, Z., & Dekel, B. (2020). Ex Vivo Expanded 3D Human Kidney Spheres Engraft Long Term and Repair Chronic Renal Injury in Mice. Cell Reports, 30(3), 852-869.e4. https://doi.org/10.1016/j.celrep.2019.12.047

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title = "Ex Vivo Expanded 3D Human Kidney Spheres Engraft Long Term and Repair Chronic Renal Injury in Mice",

abstract = "Human kidney cells quickly degenerate in culture, precluding them as a source for cell therapy for chronic kidney disease (CKD). Herein, Harari-Steinberg et al. show that upon culturing as 3D nephrospheres, human kidney cells regain their epithelial phenotype and acquire in vivo tissue-forming and reparative properties in a CKD mouse model.",

keywords = "3D culture, 5/6 nephrectomy, antifibrotic effect, autologous treatment, in vivo differentiation, kidney regeneration, kidney stem cells, mouse CKD model, nephrospheres, renal epithelial differentiation",

author = "Orit Harari-Steinberg and Dorit Omer and Yehudit Gnatek and Oren Pleniceanu and Sanja Goldberg and Osnat Cohen-Zontag and Sara Pri-Chen and {Ben Haim}, Nissim and Eli Becker and Roi Ankawa and Yaron Fuchs and Tomer Kalisky and Zohar Dotan and Benjamin Dekel",

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year = "2020",

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day = "21",

doi = "https://doi.org/10.1016/j.celrep.2019.12.047",

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Harari-Steinberg, O, Omer, D, Gnatek, Y, Pleniceanu, O, Goldberg, S, Cohen-Zontag, O, Pri-Chen, S, Ben Haim, N, Becker, E, Ankawa, R, Fuchs, Y, Kalisky, T, Dotan, Z & Dekel, B 2020, 'Ex Vivo Expanded 3D Human Kidney Spheres Engraft Long Term and Repair Chronic Renal Injury in Mice', Cell Reports, vol. 30, no. 3, pp. 852-869.e4. https://doi.org/10.1016/j.celrep.2019.12.047

TY - JOUR

T1 - Ex Vivo Expanded 3D Human Kidney Spheres Engraft Long Term and Repair Chronic Renal Injury in Mice

AU - Harari-Steinberg, Orit

AU - Omer, Dorit

AU - Gnatek, Yehudit

AU - Pleniceanu, Oren

AU - Goldberg, Sanja

AU - Cohen-Zontag, Osnat

AU - Pri-Chen, Sara

AU - Ben Haim, Nissim

AU - Becker, Eli

AU - Ankawa, Roi

AU - Fuchs, Yaron

AU - Kalisky, Tomer

AU - Dotan, Zohar

AU - Dekel, Benjamin

PY - 2020/1/21

Y1 - 2020/1/21

N2 - Human kidney cells quickly degenerate in culture, precluding them as a source for cell therapy for chronic kidney disease (CKD). Herein, Harari-Steinberg et al. show that upon culturing as 3D nephrospheres, human kidney cells regain their epithelial phenotype and acquire in vivo tissue-forming and reparative properties in a CKD mouse model.

AB - Human kidney cells quickly degenerate in culture, precluding them as a source for cell therapy for chronic kidney disease (CKD). Herein, Harari-Steinberg et al. show that upon culturing as 3D nephrospheres, human kidney cells regain their epithelial phenotype and acquire in vivo tissue-forming and reparative properties in a CKD mouse model.

KW - 3D culture

KW - 5/6 nephrectomy

KW - antifibrotic effect

KW - autologous treatment

KW - in vivo differentiation

KW - kidney regeneration

KW - kidney stem cells

KW - mouse CKD model

KW - nephrospheres

KW - renal epithelial differentiation

UR - http://www.scopus.com/inward/record.url?scp=85078001438&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.celrep.2019.12.047

DO - https://doi.org/10.1016/j.celrep.2019.12.047

M3 - مقالة

C2 - 31968258

SN - 2211-1247

VL - 30

SP - 852-869.e4

JO - Cell Reports

JF - Cell Reports

IS - 3

ER -

Ex Vivo Expanded 3D Human Kidney Spheres Engraft Long Term and Repair Chronic Renal Injury in Mice

Abstract

Keywords

All Science Journal Classification (ASJC) codes

UN SDGs

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