Evaluation of noninvasive genetic methods for Nubian ibex

Idan Goodman; Naama Shahar; Gila Kahila Bar-Gal; David Saltz; Shirli Bar-David

doi:https://doi.org/10.1007/s12686-016-0642-8

Evaluation of noninvasive genetic methods for Nubian ibex

Idan Goodman, Naama Shahar, Gila Kahila Bar-Gal, David Saltz, Shirli Bar-David

Ben-Gurion University of the Negev, The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Noninvasive genetic methods enable the sampling of natural populations while minimizing detrimental effects on them. However, noninvasive methods are marred by DNA extraction and amplification difficulties that can be mitigated by improved methodology. Past studies have shown that noninvasive genetic protocols are system specific and highlighted the importance of pilot studies in the establishment of genetic monitoring programs. We tested, using a factorial design experiment, the effect of different methods for the collection-preservation and extraction on the amplification of DNA from Nubian ibex (Capra nubiana) fecal samples. We found collection-preservation using paper bags and extraction with QIAamp^® fast DNA stool mini kit to significantly enhance success rate compared to collection-preservation in ethanol and extraction with QIAamp^® DNA mini kit. Our results will contribute to the studies of natural populations of the vulnerable C. nubiana.

Original language	American English
Pages (from-to)	181-183
Number of pages	3
Journal	Conservation Genetics Resources
Volume	9
Issue number	2
DOIs	https://doi.org/10.1007/s12686-016-0642-8
State	Published - 1 Jun 2017

Keywords

Capra nubiana
Collection-preservation
DNA extraction
Fecal DNA
Nubian ibex

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics
Genetics

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https://doi.org/10.1007/s12686-016-0642-8

Cite this

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title = "Evaluation of noninvasive genetic methods for Nubian ibex",

abstract = "Noninvasive genetic methods enable the sampling of natural populations while minimizing detrimental effects on them. However, noninvasive methods are marred by DNA extraction and amplification difficulties that can be mitigated by improved methodology. Past studies have shown that noninvasive genetic protocols are system specific and highlighted the importance of pilot studies in the establishment of genetic monitoring programs. We tested, using a factorial design experiment, the effect of different methods for the collection-preservation and extraction on the amplification of DNA from Nubian ibex (Capra nubiana) fecal samples. We found collection-preservation using paper bags and extraction with QIAamp{\textregistered} fast DNA stool mini kit to significantly enhance success rate compared to collection-preservation in ethanol and extraction with QIAamp{\textregistered} DNA mini kit. Our results will contribute to the studies of natural populations of the vulnerable C. nubiana.",

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author = "Idan Goodman and Naama Shahar and Bar-Gal, {Gila Kahila} and David Saltz and Shirli Bar-David",

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AU - Goodman, Idan

AU - Shahar, Naama

AU - Bar-Gal, Gila Kahila

AU - Saltz, David

AU - Bar-David, Shirli

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Noninvasive genetic methods enable the sampling of natural populations while minimizing detrimental effects on them. However, noninvasive methods are marred by DNA extraction and amplification difficulties that can be mitigated by improved methodology. Past studies have shown that noninvasive genetic protocols are system specific and highlighted the importance of pilot studies in the establishment of genetic monitoring programs. We tested, using a factorial design experiment, the effect of different methods for the collection-preservation and extraction on the amplification of DNA from Nubian ibex (Capra nubiana) fecal samples. We found collection-preservation using paper bags and extraction with QIAamp® fast DNA stool mini kit to significantly enhance success rate compared to collection-preservation in ethanol and extraction with QIAamp® DNA mini kit. Our results will contribute to the studies of natural populations of the vulnerable C. nubiana.

AB - Noninvasive genetic methods enable the sampling of natural populations while minimizing detrimental effects on them. However, noninvasive methods are marred by DNA extraction and amplification difficulties that can be mitigated by improved methodology. Past studies have shown that noninvasive genetic protocols are system specific and highlighted the importance of pilot studies in the establishment of genetic monitoring programs. We tested, using a factorial design experiment, the effect of different methods for the collection-preservation and extraction on the amplification of DNA from Nubian ibex (Capra nubiana) fecal samples. We found collection-preservation using paper bags and extraction with QIAamp® fast DNA stool mini kit to significantly enhance success rate compared to collection-preservation in ethanol and extraction with QIAamp® DNA mini kit. Our results will contribute to the studies of natural populations of the vulnerable C. nubiana.

KW - Capra nubiana

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Evaluation of noninvasive genetic methods for Nubian ibex

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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