Erratum: Pathogen-induced biosynthetic pathways encode defense-related molecules in bread wheat (Proceedings of the National Academy of Sciences of the United States of America (2022) 119 (e2123299119) DOI: 10.1073/pnas.2123299119)

The authors note that the structure of the product of CYP51H37 (TaHIO), compound 10 (named ellarinacin), has been revised in response to recent identification of a sodium adduct in the compound’s mass spectrum. Ellarinacin was originally assigned an exact mass of 454.34 based on an apparent [Mass + H]+ ion of 455. Identification of a sodium adduct [Mass + Na]+ of 495, demotes this 455 ion to [Mass + H – H2O]+ giving compound 10 (ellarinacin) an exact mass of 472.36. The revised assignment of the 13C & 1H NMR spectra of compound 10 (ellarinacin) consistent with a mass of 472.36 is provided in Fig. 1, along with the original and revised structure. It should also be noted that the reported [Mass + H]+ ion of 497 for the acetylated derivative of ellarinacin (acetyl-ellarinacin - the product of BdACT) is also demoted to [Mass + H – H2O]+ and the respective sodium adduct [Mass + Na]+ is observed at 537. In accordance with the revised structure of ellarinacin, the reaction catalyzed by the CYP51H37 (TaHIO) enzyme is 7,25-hydroxylation, with subsequent formation of a 3,25 ether bridge in ring A. The new figure and its legend appear below.