Abstract
Colonic homeostasis entails epithelium-lymphocyte cooperation, yet many participants in this process are unknown. We show here that epithelial microRNAs mediate the mucosa-immune system crosstalk necessary for mounting protective T helper type 2 (T(H)2) responses. Abolishing the induction of microRNA by gut-specific deletion of Dicer1 (Dicer1(Delta gut)), which encodes an enzyme involved in microRNA biogenesis, deprived goblet cells of RELM beta, a key T(H)2 antiparasitic cytokine; this predisposed the host to parasite infection. Infection of Dicer1(Delta gut) mice with helminths favored a futile T(H)1 response with hallmarks of inflammatory bowel disease. Interleukin 13 (IL-13) induced the microRNA miR-375, which regulates the expression of TSLP, a T(H)2-facilitating epithelial cytokine; this indicated a T(H)2-amplification loop. We found that miR-375 was required for RELM beta expression in vivo; miR-375-deficient mice had significantly less intestinal RELM beta, which possibly explains the greater susceptibility of Dicer1(Delta gut) mice to parasites. Our findings indicate that epithelial microRNAs are key regulators of gut homeostasis and mucosal immunity.
Original language | English |
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Pages (from-to) | 239-246 |
Number of pages | 8 |
Journal | Nature Immunology |
Volume | 12 |
Issue number | 3 |
Early online date | 30 Jan 2011 |
DOIs | |
State | Published - 1 Mar 2011 |
All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology