Epigenetic silencing of miR-145-5p contributes to brain metastasis

Sara Donzelli; Federica Mori; Teresa Bellissimo; Andrea Sacconi; Beatrice Casini; Tania Frixa; Giuseppe Roscilli; Luigi Aurisicchio; Francesco Facciolo; Alfredo Pompili; Maria Antonia Carosi; Edoardo Pescarmona; Oreste Segatto; Greg Pond; Paola Muti; Stefano Telera; Sabrina Strano; Yosef Yarden; Giovanni Blandino

doi:10.18632/oncotarget.5930

Epigenetic silencing of miR-145-5p contributes to brain metastasis

Sara Donzelli, Federica Mori, Teresa Bellissimo, Andrea Sacconi, Beatrice Casini, Tania Frixa, Giuseppe Roscilli, Luigi Aurisicchio, Francesco Facciolo, Alfredo Pompili, Maria Antonia Carosi, Edoardo Pescarmona, Oreste Segatto, Greg Pond, Paola Muti, Stefano Telera, Sabrina Strano, Yosef Yarden, Giovanni Blandino

Department of Immunology and Regenerative Biology

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Brain metastasis is a major cause of morbidity and mortality of lung cancer patients. We assessed whether aberrant expression of specific microRNAs could contribute to brain metastasis. Comparison of primary lung tumors and their matched metastatic brain disseminations identified shared patterns of several microRNAs, including common down-regulation of miR-145-5p. Down-regulation was attributed to methylation of miR-145's promoter and affiliated elevation of several protein targets, such as EGFR, OCT-4, MUC-1, c-MYC and, interestingly, tumor protein D52 (TPD52). In line with these observations, restored expression of miR-145-5p and selective depletion of individual targets markedly reduced in vitro and in vivo cancer cell migration. In aggregate, our results attribute to miR-145-5p and its direct targets pivotal roles in malignancy progression and in metastasis.

Original language	English
Pages (from-to)	35183-35201
Number of pages	19
Journal	Oncotarget
Volume	6
Issue number	34
DOIs	https://doi.org/10.18632/oncotarget.5930
State	Published - 3 Nov 2015

All Science Journal Classification (ASJC) codes

Oncology

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Donzelli, S., Mori, F., Bellissimo, T., Sacconi, A., Casini, B., Frixa, T., Roscilli, G., Aurisicchio, L., Facciolo, F., Pompili, A., Carosi, M. A., Pescarmona, E., Segatto, O., Pond, G., Muti, P., Telera, S., Strano, S., Yarden, Y., & Blandino, G. (2015). Epigenetic silencing of miR-145-5p contributes to brain metastasis. Oncotarget, 6(34), 35183-35201. https://doi.org/10.18632/oncotarget.5930

@article{4bd02d21ac8642948d5c7c7885794d1e,

title = "Epigenetic silencing of miR-145-5p contributes to brain metastasis",

abstract = "Brain metastasis is a major cause of morbidity and mortality of lung cancer patients. We assessed whether aberrant expression of specific microRNAs could contribute to brain metastasis. Comparison of primary lung tumors and their matched metastatic brain disseminations identified shared patterns of several microRNAs, including common down-regulation of miR-145-5p. Down-regulation was attributed to methylation of miR-145's promoter and affiliated elevation of several protein targets, such as EGFR, OCT-4, MUC-1, c-MYC and, interestingly, tumor protein D52 (TPD52). In line with these observations, restored expression of miR-145-5p and selective depletion of individual targets markedly reduced in vitro and in vivo cancer cell migration. In aggregate, our results attribute to miR-145-5p and its direct targets pivotal roles in malignancy progression and in metastasis.",

author = "Sara Donzelli and Federica Mori and Teresa Bellissimo and Andrea Sacconi and Beatrice Casini and Tania Frixa and Giuseppe Roscilli and Luigi Aurisicchio and Francesco Facciolo and Alfredo Pompili and Carosi, {Maria Antonia} and Edoardo Pescarmona and Oreste Segatto and Greg Pond and Paola Muti and Stefano Telera and Sabrina Strano and Yosef Yarden and Giovanni Blandino",

note = "This work was supported by AIRC to GB (AIRC 14455) and EPIGEN to GB (13/05/R/42).",

year = "2015",

month = nov,

day = "3",

doi = "10.18632/oncotarget.5930",

language = "الإنجليزيّة",

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pages = "35183--35201",

journal = "Oncotarget",

issn = "1949-2553",

publisher = "Impact Journals LLC",

number = "34",

}

Donzelli, S, Mori, F, Bellissimo, T, Sacconi, A, Casini, B, Frixa, T, Roscilli, G, Aurisicchio, L, Facciolo, F, Pompili, A, Carosi, MA, Pescarmona, E, Segatto, O, Pond, G, Muti, P, Telera, S, Strano, S, Yarden, Y & Blandino, G 2015, 'Epigenetic silencing of miR-145-5p contributes to brain metastasis', Oncotarget, vol. 6, no. 34, pp. 35183-35201. https://doi.org/10.18632/oncotarget.5930

TY - JOUR

T1 - Epigenetic silencing of miR-145-5p contributes to brain metastasis

AU - Donzelli, Sara

AU - Mori, Federica

AU - Bellissimo, Teresa

AU - Sacconi, Andrea

AU - Casini, Beatrice

AU - Frixa, Tania

AU - Roscilli, Giuseppe

AU - Aurisicchio, Luigi

AU - Facciolo, Francesco

AU - Pompili, Alfredo

AU - Carosi, Maria Antonia

AU - Pescarmona, Edoardo

AU - Segatto, Oreste

AU - Pond, Greg

AU - Muti, Paola

AU - Telera, Stefano

AU - Strano, Sabrina

AU - Yarden, Yosef

AU - Blandino, Giovanni

N1 - This work was supported by AIRC to GB (AIRC 14455) and EPIGEN to GB (13/05/R/42).

PY - 2015/11/3

Y1 - 2015/11/3

N2 - Brain metastasis is a major cause of morbidity and mortality of lung cancer patients. We assessed whether aberrant expression of specific microRNAs could contribute to brain metastasis. Comparison of primary lung tumors and their matched metastatic brain disseminations identified shared patterns of several microRNAs, including common down-regulation of miR-145-5p. Down-regulation was attributed to methylation of miR-145's promoter and affiliated elevation of several protein targets, such as EGFR, OCT-4, MUC-1, c-MYC and, interestingly, tumor protein D52 (TPD52). In line with these observations, restored expression of miR-145-5p and selective depletion of individual targets markedly reduced in vitro and in vivo cancer cell migration. In aggregate, our results attribute to miR-145-5p and its direct targets pivotal roles in malignancy progression and in metastasis.

AB - Brain metastasis is a major cause of morbidity and mortality of lung cancer patients. We assessed whether aberrant expression of specific microRNAs could contribute to brain metastasis. Comparison of primary lung tumors and their matched metastatic brain disseminations identified shared patterns of several microRNAs, including common down-regulation of miR-145-5p. Down-regulation was attributed to methylation of miR-145's promoter and affiliated elevation of several protein targets, such as EGFR, OCT-4, MUC-1, c-MYC and, interestingly, tumor protein D52 (TPD52). In line with these observations, restored expression of miR-145-5p and selective depletion of individual targets markedly reduced in vitro and in vivo cancer cell migration. In aggregate, our results attribute to miR-145-5p and its direct targets pivotal roles in malignancy progression and in metastasis.

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U2 - 10.18632/oncotarget.5930

DO - 10.18632/oncotarget.5930

M3 - مقالة

SN - 1949-2553

VL - 6

SP - 35183

EP - 35201

JO - Oncotarget

JF - Oncotarget

IS - 34

ER -

Epigenetic silencing of miR-145-5p contributes to brain metastasis

Abstract

All Science Journal Classification (ASJC) codes

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