Enzymatically incorporated genomic tags for optical mapping of DNA-binding proteins

Soohong Kim; Anna Gottfried; Ron R. Lin; Thomas Dertinger; Andrew S. Kim; Sangyoon Chung; Ryan A. Colyer; Elmar Weinhold; Shimon Weiss; Yuval Ebenstein

doi:10.1002/anie.201107714

Enzymatically incorporated genomic tags for optical mapping of DNA-binding proteins

Soohong Kim, Anna Gottfried, Ron R. Lin, Thomas Dertinger, Andrew S. Kim, Sangyoon Chung, Ryan A. Colyer, Elmar Weinhold, Shimon Weiss, Yuval Ebenstein

School of Chemistry

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

All smiles: Sequence-specific methyltransferase-induced labeling of DNA (SMILing DNA) creates a fluorescence pattern of the T7 bacteriophage genome (see picture). The pattern is visualized as a linear optical barcode showing the genomic location of individual RNA polymerases bound to the DNA. The precision of the measurement presents new opportunities for contextual genomic research on the single-molecule level.

Original language	English
Pages (from-to)	3578-3581
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	15
DOIs	https://doi.org/10.1002/anie.201107714
State	Published - 10 Apr 2012

Keywords

DNA
imaging agents
optical mapping
quantum dots
single-molecule studies

All Science Journal Classification (ASJC) codes

Catalysis
General Chemistry

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10.1002/anie.201107714

Cite this

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title = "Enzymatically incorporated genomic tags for optical mapping of DNA-binding proteins",

abstract = "All smiles: Sequence-specific methyltransferase-induced labeling of DNA (SMILing DNA) creates a fluorescence pattern of the T7 bacteriophage genome (see picture). The pattern is visualized as a linear optical barcode showing the genomic location of individual RNA polymerases bound to the DNA. The precision of the measurement presents new opportunities for contextual genomic research on the single-molecule level.",

keywords = "DNA, imaging agents, optical mapping, quantum dots, single-molecule studies",

author = "Soohong Kim and Anna Gottfried and Lin, \{Ron R.\} and Thomas Dertinger and Kim, \{Andrew S.\} and Sangyoon Chung and Colyer, \{Ryan A.\} and Elmar Weinhold and Shimon Weiss and Yuval Ebenstein",

year = "2012",

month = apr,

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doi = "10.1002/anie.201107714",

language = "الإنجليزيّة",

volume = "51",

pages = "3578--3581",

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T1 - Enzymatically incorporated genomic tags for optical mapping of DNA-binding proteins

AU - Kim, Soohong

AU - Gottfried, Anna

AU - Lin, Ron R.

AU - Dertinger, Thomas

AU - Kim, Andrew S.

AU - Chung, Sangyoon

AU - Colyer, Ryan A.

AU - Weinhold, Elmar

AU - Weiss, Shimon

AU - Ebenstein, Yuval

PY - 2012/4/10

Y1 - 2012/4/10

N2 - All smiles: Sequence-specific methyltransferase-induced labeling of DNA (SMILing DNA) creates a fluorescence pattern of the T7 bacteriophage genome (see picture). The pattern is visualized as a linear optical barcode showing the genomic location of individual RNA polymerases bound to the DNA. The precision of the measurement presents new opportunities for contextual genomic research on the single-molecule level.

AB - All smiles: Sequence-specific methyltransferase-induced labeling of DNA (SMILing DNA) creates a fluorescence pattern of the T7 bacteriophage genome (see picture). The pattern is visualized as a linear optical barcode showing the genomic location of individual RNA polymerases bound to the DNA. The precision of the measurement presents new opportunities for contextual genomic research on the single-molecule level.

KW - DNA

KW - imaging agents

KW - optical mapping

KW - quantum dots

KW - single-molecule studies

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U2 - 10.1002/anie.201107714

DO - 10.1002/anie.201107714

M3 - مقالة

C2 - 22344826

SN - 1433-7851

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EP - 3581

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 15

ER -

Enzymatically incorporated genomic tags for optical mapping of DNA-binding proteins

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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