Entropy-regularized deconvolution of cellular cryotransmission electron tomograms

Matthew Croxford; Michael Elbaum; Muthuvel Arigovindan; Zvi Kam; David Agard; Elizabeth Villa; John Sedat

doi:10.1073/pnas.2108738118

Entropy-regularized deconvolution of cellular cryotransmission electron tomograms

Matthew Croxford, Michael Elbaum, Muthuvel Arigovindan, Zvi Kam, David Agard, Elizabeth Villa, John Sedat

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularized deconvolution (ER-DC) to cryo-ET data generated from transmission electron microscopy (TEM) and reconstructed using weighted back projection (WBP). We applied deconvolution to several in situ cryo-ET datasets and assessed the results by Fourier analysis and subtomogram analysis (STA).

Original language	English
Article number	e2108738118
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	118
Issue number	50
DOIs	https://doi.org/10.1073/pnas.2108738118
State	Published - 14 Dec 2021

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title = "Entropy-regularized deconvolution of cellular cryotransmission electron tomograms",

abstract = "Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularized deconvolution (ER-DC) to cryo-ET data generated from transmission electron microscopy (TEM) and reconstructed using weighted back projection (WBP). We applied deconvolution to several in situ cryo-ET datasets and assessed the results by Fourier analysis and subtomogram analysis (STA).",

author = "Matthew Croxford and Michael Elbaum and Muthuvel Arigovindan and Zvi Kam and David Agard and Elizabeth Villa and John Sedat",

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doi = "10.1073/pnas.2108738118",

language = "الإنجليزيّة",

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T1 - Entropy-regularized deconvolution of cellular cryotransmission electron tomograms

AU - Croxford, Matthew

AU - Elbaum, Michael

AU - Arigovindan, Muthuvel

AU - Kam, Zvi

AU - Agard, David

AU - Villa, Elizabeth

AU - Sedat, John

PY - 2021/12/14

Y1 - 2021/12/14

N2 - Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularized deconvolution (ER-DC) to cryo-ET data generated from transmission electron microscopy (TEM) and reconstructed using weighted back projection (WBP). We applied deconvolution to several in situ cryo-ET datasets and assessed the results by Fourier analysis and subtomogram analysis (STA).

AB - Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularized deconvolution (ER-DC) to cryo-ET data generated from transmission electron microscopy (TEM) and reconstructed using weighted back projection (WBP). We applied deconvolution to several in situ cryo-ET datasets and assessed the results by Fourier analysis and subtomogram analysis (STA).

UR - http://www.scopus.com/inward/record.url?scp=85121030858&partnerID=8YFLogxK

U2 - 10.1073/pnas.2108738118

DO - 10.1073/pnas.2108738118

M3 - مقالة

C2 - 34876518

SN - 0027-8424

VL - 118

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 50

M1 - e2108738118

ER -

Entropy-regularized deconvolution of cellular cryotransmission electron tomograms

Abstract

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