Enhanced infarct stabilization and neovascularization mediated by VEGF-loaded PEGylated fibrinogen hydrogel in a rodent myocardial infarction model

Abdul Jalil Rufaihah; Srirangam Ramanujam Vaibavi; Marian Plotkin; Jiayi Shen; Venkateswaran Nithya; Jing Wang; Dror Seliktar; Theodoros Kofidis

doi:https://doi.org/10.1016/j.biomaterials.2013.07.031

Enhanced infarct stabilization and neovascularization mediated by VEGF-loaded PEGylated fibrinogen hydrogel in a rodent myocardial infarction model

Abdul Jalil Rufaihah, Srirangam Ramanujam Vaibavi, Marian Plotkin, Jiayi Shen, Venkateswaran Nithya, Jing Wang, Dror Seliktar, Theodoros Kofidis

Biomedical Engineering

Technion - Israel Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Most tissue engineering therapies require biomaterials that are able to induce an angiogenic response to support tissue regeneration. In addition angiogenic growth factor signaling plays an essential role in controlling the process of angiogenesis and matrices have the potential of regulating the concentration of growth factors within the cellular microenvironment. Here we demonstrated myocardial protection and improved post-infarct vascularization of the infarcted hearts using a biosynthetic injectable hydrogel consisting of polyethylene glycol and fibrinogen (PEG-fibrinogen) loaded with vascular endothelial growth factor-A (VEGF-A). Our data revealed PEG-fibrinogen hydrogel was able to store and release VEGF-A in a sustained and controlled fashion. Upon injection after coronary artery ligation, the VEGF-loaded hydrogel significantly improved arteriogenesis and cardiac performance at 4 weeks post-infarction. The results support the future application of PEG-fibrinogen for regulating growth factor signaling in cellular microenvironment and may demonstrates a new strategy for cardiovascular repair with potential for future clinical applications.

Original language	English
Pages (from-to)	8195-8202
Number of pages	8
Journal	Biomaterials
Volume	34
Issue number	33
DOIs	https://doi.org/10.1016/j.biomaterials.2013.07.031
State	Published - Nov 2013

Keywords

Cardiac tissue engineering
Growth factors
Hydrogel
In vivo test

All Science Journal Classification (ASJC) codes

Mechanics of Materials
Ceramics and Composites
Bioengineering
Biophysics
Biomaterials

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

https://doi.org/10.1016/j.biomaterials.2013.07.031

Cite this

@article{ad3a1d30074c4738b71d1e6edffedd7c,

title = "Enhanced infarct stabilization and neovascularization mediated by VEGF-loaded PEGylated fibrinogen hydrogel in a rodent myocardial infarction model",

abstract = "Most tissue engineering therapies require biomaterials that are able to induce an angiogenic response to support tissue regeneration. In addition angiogenic growth factor signaling plays an essential role in controlling the process of angiogenesis and matrices have the potential of regulating the concentration of growth factors within the cellular microenvironment. Here we demonstrated myocardial protection and improved post-infarct vascularization of the infarcted hearts using a biosynthetic injectable hydrogel consisting of polyethylene glycol and fibrinogen (PEG-fibrinogen) loaded with vascular endothelial growth factor-A (VEGF-A). Our data revealed PEG-fibrinogen hydrogel was able to store and release VEGF-A in a sustained and controlled fashion. Upon injection after coronary artery ligation, the VEGF-loaded hydrogel significantly improved arteriogenesis and cardiac performance at 4 weeks post-infarction. The results support the future application of PEG-fibrinogen for regulating growth factor signaling in cellular microenvironment and may demonstrates a new strategy for cardiovascular repair with potential for future clinical applications.",

keywords = "Cardiac tissue engineering, Growth factors, Hydrogel, In vivo test",

author = "Rufaihah, {Abdul Jalil} and Vaibavi, {Srirangam Ramanujam} and Marian Plotkin and Jiayi Shen and Venkateswaran Nithya and Jing Wang and Dror Seliktar and Theodoros Kofidis",

note = "Funding Information: The authors would like to acknowledge funding support from National Research Foundation (NRF)-Technion-NUS Grant for Regenerative Medicine Initiative in Cardiac Restoration Therapy.",

year = "2013",

month = nov,

doi = "https://doi.org/10.1016/j.biomaterials.2013.07.031",

language = "الإنجليزيّة",

volume = "34",

pages = "8195--8202",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier Ltd.",

number = "33",

}

TY - JOUR

T1 - Enhanced infarct stabilization and neovascularization mediated by VEGF-loaded PEGylated fibrinogen hydrogel in a rodent myocardial infarction model

AU - Rufaihah, Abdul Jalil

AU - Vaibavi, Srirangam Ramanujam

AU - Plotkin, Marian

AU - Shen, Jiayi

AU - Nithya, Venkateswaran

AU - Wang, Jing

AU - Seliktar, Dror

AU - Kofidis, Theodoros

N1 - Funding Information: The authors would like to acknowledge funding support from National Research Foundation (NRF)-Technion-NUS Grant for Regenerative Medicine Initiative in Cardiac Restoration Therapy.

PY - 2013/11

Y1 - 2013/11

N2 - Most tissue engineering therapies require biomaterials that are able to induce an angiogenic response to support tissue regeneration. In addition angiogenic growth factor signaling plays an essential role in controlling the process of angiogenesis and matrices have the potential of regulating the concentration of growth factors within the cellular microenvironment. Here we demonstrated myocardial protection and improved post-infarct vascularization of the infarcted hearts using a biosynthetic injectable hydrogel consisting of polyethylene glycol and fibrinogen (PEG-fibrinogen) loaded with vascular endothelial growth factor-A (VEGF-A). Our data revealed PEG-fibrinogen hydrogel was able to store and release VEGF-A in a sustained and controlled fashion. Upon injection after coronary artery ligation, the VEGF-loaded hydrogel significantly improved arteriogenesis and cardiac performance at 4 weeks post-infarction. The results support the future application of PEG-fibrinogen for regulating growth factor signaling in cellular microenvironment and may demonstrates a new strategy for cardiovascular repair with potential for future clinical applications.

AB - Most tissue engineering therapies require biomaterials that are able to induce an angiogenic response to support tissue regeneration. In addition angiogenic growth factor signaling plays an essential role in controlling the process of angiogenesis and matrices have the potential of regulating the concentration of growth factors within the cellular microenvironment. Here we demonstrated myocardial protection and improved post-infarct vascularization of the infarcted hearts using a biosynthetic injectable hydrogel consisting of polyethylene glycol and fibrinogen (PEG-fibrinogen) loaded with vascular endothelial growth factor-A (VEGF-A). Our data revealed PEG-fibrinogen hydrogel was able to store and release VEGF-A in a sustained and controlled fashion. Upon injection after coronary artery ligation, the VEGF-loaded hydrogel significantly improved arteriogenesis and cardiac performance at 4 weeks post-infarction. The results support the future application of PEG-fibrinogen for regulating growth factor signaling in cellular microenvironment and may demonstrates a new strategy for cardiovascular repair with potential for future clinical applications.

KW - Cardiac tissue engineering

KW - Growth factors

KW - Hydrogel

KW - In vivo test

UR - http://www.scopus.com/inward/record.url?scp=84881662403&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.biomaterials.2013.07.031

DO - https://doi.org/10.1016/j.biomaterials.2013.07.031

M3 - مقالة

SN - 0142-9612

VL - 34

SP - 8195

EP - 8202

JO - Biomaterials

JF - Biomaterials

IS - 33

ER -

Enhanced infarct stabilization and neovascularization mediated by VEGF-loaded PEGylated fibrinogen hydrogel in a rodent myocardial infarction model

Abstract

Keywords

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this