Encapsulation of pseudomonas sp. ADP cells in electrospun microtubes for atrazine bioremediation

Shiri Klein, Ron Avrahami, Eyal Zussman, Michael Beliavski, Sheldon Tarre, Michal Green

Research output: Contribution to journalArticlepeer-review

Abstract

Electrospun hollow polymeric microfibers (microtubes) were evaluated as an encapsulation method for the atrazine degrading bacterium Pseudomonas sp. ADP. Pseudomonas sp. ADP cells were successfully incorporated in a formulation containing a core solution of polyethylene oxide dissolved in water and spun with an outer shell solution made of polycaprolactone and polyethylene glycol dissolved in a chloroform and dimethylformamide. The resulting microtubes, collected as mats, were partially collapsed with a ribbon-like structure. Following encapsulation, the atrazine degradation rate was low (0.03 ± 0.01 mg atrazine/h/g fiber) indicating that the electrospinning process negatively affected cell activity. Atrazine degradation was restored to 0.5 ± 0.1 mg atrazine/ h/g fiber by subjecting the microtubes to a period of growth. After 3 and 7 days growth periods, encapsulated cells were able to remove 20.6 ± 3 and 47.6 ± 5.9 mg atrazine/g mat, respectively, in successive batches under non-growth conditions (with no additional electron donor) until atrazine was detected in the medium. The loss of atrazine degrading capacity was regained following an additional cell-growth period..

Original languageEnglish
Pages (from-to)1605-1613
Number of pages9
JournalJournal of Industrial Microbiology and Biotechnology
Volume39
Issue number11
DOIs
StatePublished - Nov 2012

Keywords

  • Atrazine
  • Bioremediation
  • Cell encapsulation
  • Electrospinning
  • Microtubes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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