TY - JOUR
T1 - Electronic modulation of biochemical signal generation
AU - Gordonov, Tanya
AU - Kim, Eunkyoung
AU - Cheng, Yi
AU - Ben-Yoav, Hadar
AU - Ghodssi, Reza
AU - Rubloff, Gary
AU - Yin, Jun Jie
AU - Payne, Gregory F.
AU - Bentley, William E.
N1 - Funding Information: The authors thank the UMD Fischell Department of Bioengineering Core FACS Facility for assistance with FACS data collection and the UMD Nanocenter for providing workspace and tools for electrode fabrication and ICP-EOS measurements. The authors thank Y. Zhou of the UMD Department of Nutrition and Food Science for help with EPR measurements. Financial support for this work was provided by the Defense Threat Reduction Agency (HDTRA1-13-0037), the National Science Foundation (no. 1160005 to WEB, no. 1264509 to HO Sintim) and the RWD Foundation.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Microelectronic devices that contain biological components are typically used to interrogate biology1,2 rather than control biological function. Patterned assemblies of proteins and cells have, however, been used for in vitro metabolic engineering3-7, where coordinated biochemical pathways allow cell metabolism to be characterized and potentially controlled8 on a chip. Such devices form part of technologies that attempt to recreate animal and human physiological functions on a chip 9 and could be used to revolutionize drug development10. These ambitious goals will, however, require new biofabrication methodologies that help connect microelectronics and biological systems11,12 and yield new approaches to device assembly and communication. Here, we report the electrically mediated assembly, interrogation and control of a multi-domain fusion protein that produces a bacterial signalling molecule. The biological system can be electrically tuned using a natural redox molecule, and its biochemical response is shown to provide the signalling cues to drive bacterial population behaviour. We show that the biochemical output of the system correlates with the electrical input charge, which suggests that electrical inputs could be used to control complex on-chip biological processes.
AB - Microelectronic devices that contain biological components are typically used to interrogate biology1,2 rather than control biological function. Patterned assemblies of proteins and cells have, however, been used for in vitro metabolic engineering3-7, where coordinated biochemical pathways allow cell metabolism to be characterized and potentially controlled8 on a chip. Such devices form part of technologies that attempt to recreate animal and human physiological functions on a chip 9 and could be used to revolutionize drug development10. These ambitious goals will, however, require new biofabrication methodologies that help connect microelectronics and biological systems11,12 and yield new approaches to device assembly and communication. Here, we report the electrically mediated assembly, interrogation and control of a multi-domain fusion protein that produces a bacterial signalling molecule. The biological system can be electrically tuned using a natural redox molecule, and its biochemical response is shown to provide the signalling cues to drive bacterial population behaviour. We show that the biochemical output of the system correlates with the electrical input charge, which suggests that electrical inputs could be used to control complex on-chip biological processes.
UR - http://www.scopus.com/inward/record.url?scp=84905818967&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/nnano.2014.151
DO - https://doi.org/10.1038/nnano.2014.151
M3 - Article
SN - 1748-3387
VL - 9
SP - 605
EP - 610
JO - Nature Nanotechnology
JF - Nature Nanotechnology
IS - 8
ER -