Electron transfer rate analysis of a site-specifically wired copper oxidase

Orr Schlesinger; Mor Pasi; Rambabu Dandela; Michael M. Meijler; Lital Alfonta

doi:10.1039/c8cp00041g

Electron transfer rate analysis of a site-specifically wired copper oxidase

Orr Schlesinger, Mor Pasi, Rambabu Dandela, Michael M. Meijler, Lital Alfonta

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

Electron transfer kinetic parameters of site-specifically wired copper oxidase were investigated. The enzyme's orientation towards the electrode was controlled by incorporation of propargyl-l-lysine as a site-specific anchoring point. Herein, we demonstrate the importance of immobilization orientation and how it affects electron transfer efficiency and kinetics to each of the enzyme's two active sites.

Original language	American English
Pages (from-to)	6159-6166
Number of pages	8
Journal	Physical Chemistry Chemical Physics
Volume	20
Issue number	9
DOIs	https://doi.org/10.1039/c8cp00041g
State	Published - 1 Jan 2018

All Science Journal Classification (ASJC) codes

General Physics and Astronomy
Physical and Theoretical Chemistry

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10.1039/c8cp00041g

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title = "Electron transfer rate analysis of a site-specifically wired copper oxidase",

abstract = "Electron transfer kinetic parameters of site-specifically wired copper oxidase were investigated. The enzyme's orientation towards the electrode was controlled by incorporation of propargyl-l-lysine as a site-specific anchoring point. Herein, we demonstrate the importance of immobilization orientation and how it affects electron transfer efficiency and kinetics to each of the enzyme's two active sites.",

author = "Orr Schlesinger and Mor Pasi and Rambabu Dandela and Meijler, \{Michael M.\} and Lital Alfonta",

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AU - Pasi, Mor

AU - Dandela, Rambabu

AU - Meijler, Michael M.

AU - Alfonta, Lital

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Electron transfer kinetic parameters of site-specifically wired copper oxidase were investigated. The enzyme's orientation towards the electrode was controlled by incorporation of propargyl-l-lysine as a site-specific anchoring point. Herein, we demonstrate the importance of immobilization orientation and how it affects electron transfer efficiency and kinetics to each of the enzyme's two active sites.

AB - Electron transfer kinetic parameters of site-specifically wired copper oxidase were investigated. The enzyme's orientation towards the electrode was controlled by incorporation of propargyl-l-lysine as a site-specific anchoring point. Herein, we demonstrate the importance of immobilization orientation and how it affects electron transfer efficiency and kinetics to each of the enzyme's two active sites.

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U2 - 10.1039/c8cp00041g

DO - 10.1039/c8cp00041g

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EP - 6166

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

IS - 9

ER -

Electron transfer rate analysis of a site-specifically wired copper oxidase

Abstract

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