@inbook{43ea0c036a2f4b5fab8396be4bb97fcb,
title = "Electrofusion of giant unilamellar vesicles to cells",
abstract = "We describe a method for electroporation-induced fusion of giant unilamellar vesicles (GUVs) with the plasma membrane of adherent cells. Using this method, the area of the cell membrane can be abruptly increased and various lipids can be introduced into the membrane. The process involves two steps: (1) the formation of GUVs with controlled membrane composition and (2) electrofusion of the GUVs to living cells using an electroporator for adherent cells. We demonstrate the technique on fish epithelial keratocytes and human foreskin fibroblasts, and discuss the influence of the composition of the GUVs on the fusion process.",
keywords = "Cell biomechanics, Cell membrane, Cell motility, Electrofusion, Giant vesicles, Membrane area",
author = "{Raz-Ben Aroush}, Dikla and Shlomit Yehudai-Resheff and Kinneret Keren",
note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",
year = "2015",
doi = "https://doi.org/10.1016/bs.mcb.2014.11.005",
language = "الإنجليزيّة",
isbn = "9780128011034",
volume = "125",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "409--422",
editor = "Ewa Paluch",
booktitle = "Biophysical Methods in Cell Biology",
}