Electrochemical determination of hydroxyurea in a complex biological matrix using mos2-modified electrodes and chemometrics

Remi Cazelles, Rajendra P. Shukla, Russell E. Ware, Alexander A. Vinks, Hadar Ben-Yoav

Research output: Contribution to journalArticlepeer-review


Hydroxyurea, an oral medication with important clinical benefits in the treatment of sickle cell anemia, can be accurately determined in plasma with a transition metal dichalcogenide-based electrochemical sensor. We used a two-dimensional molybdenum sulfide material (MoS2) selectively electrodeposited on a polycrystalline gold electrode via tailored waveform polarization in the gold electrical double layer formation region. The electro-activity of the modified electrode depends on the electrical waveform parameters used to electro-deposit MoS2. The concomitant oxidation of the MoS2 material during its electrodeposition allows for the tuning of the sensor’s specificity. Chemometrics, utilizing mathematical procedures such as principal component analysis and multivariable partial least square regression, were used to process the electrochemical data generated at the bare and the modified electrodes, thus allowing the hydroxyurea concentrations to be predicted in human plasma. A limit-of-detection of 22 nM and a sensitivity of 37 nA cm−2 µM−1 were found to be suitable for pharmaceutical and clinical applications.

Original languageAmerican English
Article number6
Pages (from-to)1-17
Number of pages17
Issue number1
StatePublished - 1 Jan 2021


  • Chemometrics
  • Electroanalytical chemistry
  • Electronic tongue
  • Hydroxyurea
  • Molybdenum sulfide
  • Sickle cell disease

All Science Journal Classification (ASJC) codes

  • General Biochemistry,Genetics and Molecular Biology
  • Medicine (miscellaneous)


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