Electrochemical detection of superoxide anions in HeLa cells by using two enzyme-free sensors prepared from ZIF-8-derived carbon nanomaterials

Yufei Li, Huanhuan Zhang, Xuan Cai, Hongli Zhao, Shlomo Magdassi, Minbo Lan

Research output: Contribution to journalArticlepeer-review

Abstract

Two kinds of carbon-based nanozymes were constructed from the same precursor of zeolitic imidazolate framework-8 (ZIF-8) for O2•− determination. Hollow carbon cubic nanomaterial (labelled as HCC) was obtained by chemically etching ZIF-8 with tannic acid and a subsequent calcination. A porous carbon cubic nanomaterial (labelled as PCC) was prepared by directly pyrolysis. Then HCC and PCC were immobilized on the surface of screen printed carbon electrodes (SPCE), fabricating HCC and PCC modified electrodes (denoted as HCC/SPCE and PCC/SPCE). HCC/SPCE, best operated at −0.5 V (vs. Ag/AgCl), has a sensitivity of 6.55 × 102 nA μM−1 cm−2 with a detection limit of 207 nM (at S/N = 3) for O2•− sensing. And PCC/SPCE, best operated at −0.4 V (vs. Ag/AgCl), exhibited a superior performance for O2•− detection with a sensitivity of 1.14 × 103 nA μM−1 cm−2 and a low detection limit of 140 nM (at S/N = 3). The two sensors possess excellent reproducibility and stability. They were used to sense O2•− released from HeLa cells. [Figure not available: see fulltext.].

Original languageAmerican English
Article number370
JournalMicrochimica Acta
Volume186
Issue number6
DOIs
StatePublished - 1 Jun 2019

Keywords

  • Carbon-based nanozymes
  • Metal-organic frameworks
  • Non-enzymatic sensors
  • Screen printed carbon electrodes
  • Zeolitic imidazolate framework

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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