@article{529e52ac128d48aa82552731db49da08,
title = "Efficiency and specificity in microRNA biogenesis",
abstract = "Primary microRNA cleavage by the Droshag-Dgcr8 'Microprocessor' complex is critical for microRNA biogenesis. Yet, the Microprocessor may also cleave other nuclear RNAs in a nonspecific manner. We studied Microprocessor function using mathematical modeling and experiments in mouse and human tissues. We found that the autoregulatory feedback on Microprocessor expression is instrumental for balancing the efficiency and specificity of its activity by effectively tuning Microprocessor levels to those of its pri-miRNA substrate.",
author = "Omer Barad and Mati Mann and Elik Chapnik and Archana Shenoy and Robert Blelloch and Naama Barkai and Eran Hornstein",
note = "Israel Science Foundation; Merck; Lilly Fulop; Wolfson family charitable trust; Azrieli Foundation; European Research Council; Helen and Martin Kimmel Award for Innovative Investigation; California Institute for Regenerative Medicine [RN2-00906-1]; US National Institutes of Health [R01 NS057221]We thank G.J. Hannon of the Cold Spring Harbor Laboratory (CSHL), F.V. Karginov (CSHL), D.R. Littman (New York University School of Medicine), R. Agami (Het Nederlands Kanker Instituut-Antoni van Leeuwenhoek) and N.V. Kim (Seoul National University) for reagents. We thank N. Shomron, D. Sprintzak, A. Horovitz, D.S. Tawfik and A. Eldar for comments on the manuscript. This work was supported by grants to E.H. from the Israel Science Foundation; the estates of F. Blau, L. Asseof, F. Sherr, C. Vener, J. Benattar, D. Francouer (Merck), Lilly Fulop, N. Baltor and M. Halphen; and the Wolfson family charitable trust. O.B. was supported by the Azrieli Foundation. E.H. is the incumbent of the Dr. Sydney Brenner Chair and the Helen and Milton A. Kimmelman Career Development Chair. N.B. is the incumbent of the Lorna Greenberg Scherzer Professorial Chair and was supported by a European Research Council Advanced Grant and a Helen and Martin Kimmel Award for Innovative Investigation. R.B. was supported by the California Institute for Regenerative Medicine (New Faculty Award RN2-00906-1) and the US National Institutes of Health (R01 NS057221).",
year = "2012",
month = jun,
doi = "https://doi.org/10.1038/nsmb.2293",
language = "الإنجليزيّة",
volume = "19",
pages = "650--652",
journal = "Nature Structural & Molecular Biology",
issn = "1545-9993",
publisher = "Nature Research",
number = "6",
}