TY - JOUR
T1 - Effect of suided tissue regeneration on newly formed Bone and cementum in periapical tissue healing after endodontic surgery
T2 - An in vivo study in the cat
AU - Artzi, Zvi
AU - Wasersprung, Nadav
AU - Weinreb, Miron
AU - Steigmann, Marius
AU - Prasad, Hari S.
AU - Tsesis, Igor
N1 - Funding Information: Supported by a grant from Botiss Biomaterials GmbH Germany and by the Alpha Omega Research Fund , Tel Aviv University.
PY - 2012/2
Y1 - 2012/2
N2 - Introduction: The purpose of this study was to evaluate the influence of anorganic bovine bone as a grafted biomaterial on newly formed bone and cementum in periapical regions after surgical endodontic treatment in cats. Methods: After inducing apical periodontitis in 9 cats, root canal and surgical endodontic treatment were performed on 72 roots of first and second maxillary premolars. Bone defects were treated with biomaterial particles + a membrane, biomaterial only, a membrane only, or left unfilled (control). Histomorphometry on nondecalcified sections were performed at 3 and 6 months after surgery. Analysis of variance with repeated measures was used within 2 and 3 subject factors to analyze newly formed bone, cementum, biomaterial conduction, and resorption. Results: At each time period, bone formation was greater at the grafted membrane-protected sites than in the grafted-unprotected sites. At 6 months, the bone area fraction at membrane nongrafted sites was greater than in the grafted-protected sites. The new cementum was significantly greater at 6 months than at 3 months and greater at the grafted membrane-protected sites over the unprotected ones at 6 months. Statistically, the grafted biomaterial, the membrane, and the time contributed significantly to the amount of new bone (P <.05) with no significant interaction. Biomaterial osteoconduction was significantly affected by the time. All 3 variables showed a significant interaction on new cementum. Conclusions: There was significantly more bone formation after surgical endodontic treatment when membrane and bone grafts were used as compared with bone grafts only or unfilled control sites. However, it appears that the key factor to the enhanced tissue regeneration is the membrane and not the grafted biomaterial.
AB - Introduction: The purpose of this study was to evaluate the influence of anorganic bovine bone as a grafted biomaterial on newly formed bone and cementum in periapical regions after surgical endodontic treatment in cats. Methods: After inducing apical periodontitis in 9 cats, root canal and surgical endodontic treatment were performed on 72 roots of first and second maxillary premolars. Bone defects were treated with biomaterial particles + a membrane, biomaterial only, a membrane only, or left unfilled (control). Histomorphometry on nondecalcified sections were performed at 3 and 6 months after surgery. Analysis of variance with repeated measures was used within 2 and 3 subject factors to analyze newly formed bone, cementum, biomaterial conduction, and resorption. Results: At each time period, bone formation was greater at the grafted membrane-protected sites than in the grafted-unprotected sites. At 6 months, the bone area fraction at membrane nongrafted sites was greater than in the grafted-protected sites. The new cementum was significantly greater at 6 months than at 3 months and greater at the grafted membrane-protected sites over the unprotected ones at 6 months. Statistically, the grafted biomaterial, the membrane, and the time contributed significantly to the amount of new bone (P <.05) with no significant interaction. Biomaterial osteoconduction was significantly affected by the time. All 3 variables showed a significant interaction on new cementum. Conclusions: There was significantly more bone formation after surgical endodontic treatment when membrane and bone grafts were used as compared with bone grafts only or unfilled control sites. However, it appears that the key factor to the enhanced tissue regeneration is the membrane and not the grafted biomaterial.
KW - Apical periodontitis
KW - apicectomy
KW - bovine bone mineral
KW - guided tissue regeneration
KW - surgical endodontics
KW - xenograft
UR - http://www.scopus.com/inward/record.url?scp=84855941340&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.joen.2011.10.002
DO - https://doi.org/10.1016/j.joen.2011.10.002
M3 - مقالة
C2 - 22244630
SN - 0099-2399
VL - 38
SP - 163
EP - 169
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 2
ER -