TY - JOUR
T1 - Effect of b-Alanine Supplementation on Monocyte Recruitment and Cognition During a 24-Hour Simulated Military Operation
AU - Wells, Adam J.
AU - Varanoske, Alyssa N.
AU - Coker, Nicholas A.
AU - Kozlowski, Gregory J.
AU - Frosti, Cheyanne L.
AU - Boffey, David
AU - Harat, Idan
AU - Jahani, Shiva
AU - Gepner, Yftach
AU - Hoffman, Jay R.
N1 - Publisher Copyright: © 2020 National Strength and Conditioning Association.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Wells, AJ, Varanoske, AN, Coker, NA, Kozlowski, GJ, Frosti, CL, Boffey, D, Harat, I, Jahani, S, Gepner, Y, and Hoffman, JR. Effect of b-alanine supplementation on monocyte recruitment and cognition during a 24-hour simulated military operation. J Strength Cond Res 34(11): 3042–3054, 2020—Sustained military operations (SUSOPs) result in psychological stress and cognitive dysfunction, which may be related to the recruitment of classical monocytes into the brain. This study examined the effect of beta-alanine (BA) on cognition and monocyte recruitment during a simulated 24-hour SUSOP. Nineteen healthy men ingested 12-g/d BA or placebo for 14 days before an SUSOP. Monocyte chemoattractant protein-1 (MCP-1), C-C chemokine receptor-2 (CCR2), and macrophage-1-antigen (CD11b) expression were assessed through multiplex assay and flow cytometry. Psychological stress and cognition were assessed through Automated Neuropsychological Assessment Metrics (ANAM). A composite measure of cognition (COGcomp) was generated from throughput scores extracted from 7 ANAM cognitive tests. Assessments occurred at baseline (0H), 12 hours (12H), 18 hours (18H), and 24 hours (24H). Significance was accepted at p # 0.05. No significant effect of BA was noted for any variable (p’s . 0.05). The frequency and severity of symptoms of psychological stress increased significantly at 18 and 24H compared with 0 and 12H (p’s, 0.05). COGcomp decreased significantly at 18 and 24H compared with 0 and 12H (p’s # 0.001). MCP-1 peaked at 18H was significantly lower at 24H compared with 18H but remained elevated at 24H compared with 0H (p’s, 0.001). CCR2 expression was significantly lower at 12 (p 5 0.031), 18, and 24H (p’s, 0.001). CD11b expression was significantly higher at 12H (p 5 0.039) and 24H (p’s 5 0.003). MCP-1 was negatively associated with COGcomp (b 5 20.395, p 5 0.002, r2 5 0.174). Neither CCR2 or CD11b was related to COGcomp (p’s . 0.05). Cognitive dysfunction during SUSOPs is related to serum concentrations of MCP-1 but is not influenced by BA supplementation.
AB - Wells, AJ, Varanoske, AN, Coker, NA, Kozlowski, GJ, Frosti, CL, Boffey, D, Harat, I, Jahani, S, Gepner, Y, and Hoffman, JR. Effect of b-alanine supplementation on monocyte recruitment and cognition during a 24-hour simulated military operation. J Strength Cond Res 34(11): 3042–3054, 2020—Sustained military operations (SUSOPs) result in psychological stress and cognitive dysfunction, which may be related to the recruitment of classical monocytes into the brain. This study examined the effect of beta-alanine (BA) on cognition and monocyte recruitment during a simulated 24-hour SUSOP. Nineteen healthy men ingested 12-g/d BA or placebo for 14 days before an SUSOP. Monocyte chemoattractant protein-1 (MCP-1), C-C chemokine receptor-2 (CCR2), and macrophage-1-antigen (CD11b) expression were assessed through multiplex assay and flow cytometry. Psychological stress and cognition were assessed through Automated Neuropsychological Assessment Metrics (ANAM). A composite measure of cognition (COGcomp) was generated from throughput scores extracted from 7 ANAM cognitive tests. Assessments occurred at baseline (0H), 12 hours (12H), 18 hours (18H), and 24 hours (24H). Significance was accepted at p # 0.05. No significant effect of BA was noted for any variable (p’s . 0.05). The frequency and severity of symptoms of psychological stress increased significantly at 18 and 24H compared with 0 and 12H (p’s, 0.05). COGcomp decreased significantly at 18 and 24H compared with 0 and 12H (p’s # 0.001). MCP-1 peaked at 18H was significantly lower at 24H compared with 18H but remained elevated at 24H compared with 0H (p’s, 0.001). CCR2 expression was significantly lower at 12 (p 5 0.031), 18, and 24H (p’s, 0.001). CD11b expression was significantly higher at 12H (p 5 0.039) and 24H (p’s 5 0.003). MCP-1 was negatively associated with COGcomp (b 5 20.395, p 5 0.002, r2 5 0.174). Neither CCR2 or CD11b was related to COGcomp (p’s . 0.05). Cognitive dysfunction during SUSOPs is related to serum concentrations of MCP-1 but is not influenced by BA supplementation.
KW - CCR2
KW - CD11b
KW - MCP-1
KW - PLS-SEM
KW - classical monocytes
KW - military
UR - http://www.scopus.com/inward/record.url?scp=85094816411&partnerID=8YFLogxK
U2 - https://doi.org/10.1519/JSC.0000000000003809
DO - https://doi.org/10.1519/JSC.0000000000003809
M3 - Article
C2 - 33105353
SN - 1064-8011
VL - 34
SP - 3042
EP - 3054
JO - Journal of Strength and Conditioning Research
JF - Journal of Strength and Conditioning Research
IS - 11
ER -