TY - JOUR
T1 - DUXO, a novel double homeobox transcription factor, is a regulator of the gastrula organizer in human embryonic stem cells
AU - Sharon, Nadav
AU - Mor, Ishay
AU - Zahavi, Eden
AU - Benvenisty, Nissim
N1 - Funding Information: We thank Tamar Golan-Lev for her assistance with the graphic design, and Dr. Jonathan Nissenbaum for critically reviewing the manuscript. N.B. is the Herbert Cohn Chair in Cancer Research. This research was supported by The Legacy Heritage Biomedical Science Partnership Program of the Israel Science Foundation (Grant No. 943/09 ) and by the Centers of Excellence Legacy Heritage Biomedical Science Partnership (Grant No. 1801/10 ).
PY - 2012/11
Y1 - 2012/11
N2 - Human embryonic stem cells differentiate into gastrula organizer cells that express typical markers and induce secondary axes when injected into frog embryos. Here, we report that these human organizer cells express DUXO (DUX of the Organizer), a novel member of the double-homeobox (DUX) family of transcription factors, a group of genes unique to placental mammals. Both of DUXO's homeodomains share high similarity with those of Siamois and Twin, the initial inducers of the amphibian gastrula organizer. DUXO overexpression in human embryoid bodies induces organizer related genes, whereas its knock down hampers formation of the organizer and its derivatives. Finally, we show that DUXO regulates GOOSECOID, the canonical organizer marker, in a direct manner, suggesting that DUXO is a major regulator of human organizer formation.
AB - Human embryonic stem cells differentiate into gastrula organizer cells that express typical markers and induce secondary axes when injected into frog embryos. Here, we report that these human organizer cells express DUXO (DUX of the Organizer), a novel member of the double-homeobox (DUX) family of transcription factors, a group of genes unique to placental mammals. Both of DUXO's homeodomains share high similarity with those of Siamois and Twin, the initial inducers of the amphibian gastrula organizer. DUXO overexpression in human embryoid bodies induces organizer related genes, whereas its knock down hampers formation of the organizer and its derivatives. Finally, we show that DUXO regulates GOOSECOID, the canonical organizer marker, in a direct manner, suggesting that DUXO is a major regulator of human organizer formation.
UR - http://www.scopus.com/inward/record.url?scp=84866481598&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.scr.2012.08.003
DO - https://doi.org/10.1016/j.scr.2012.08.003
M3 - مقالة
C2 - 23010573
SN - 1873-5061
VL - 9
SP - 261
EP - 269
JO - Stem Cell Research
JF - Stem Cell Research
IS - 3
ER -