DTR-mediated conditional cell ablationProgress and challenges

Christiane Ruedl, Steffen Jung

Research output: Contribution to journalEditorialpeer-review


Cell ablation is a valuable complement to mutagenesis for experimentally defining specific cell functions in physiology and pathophysiology in small animal models. One of the most popular ablation strategies involves transgenic expression of a primate diphtheria toxin receptor (DTR) on murine cells that are otherwise resistant to the bacterial exotoxin. The efforts of many laboratories using the DTR approach over the years have yielded numerous valuable insights into specific cell functions. Here, we will discuss the technical aspects of the DTR approach, including the strengths, pitfalls, and future strategies to overcome the shortcomings, highlighting a recent paper published in the European Journal of Immunology [El Hachem etal. Eur. J. Immunol. 2018 ]. A particular focus will be given to the application of DTR approach to decipher in vivo functions of the murine myeloid cell compartment.

Original languageEnglish
Pages (from-to)1114-1119
Number of pages6
JournalEuropean Journal of Immunology
Issue number7
StatePublished - 5 Jul 2018


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