Drought tolerance mechanisms and aquaporin expression of wild vs. cultivated pear tree species in the field

Indira Paudel; Hadas Gerbi; Annat Zisovich; Gal Sapir; Shifra Ben-Dor; Vlad Brumfeld; Tamir Klein

doi:https://doi.org/10.1016/j.envexpbot.2019.103832

Drought tolerance mechanisms and aquaporin expression of wild vs. cultivated pear tree species in the field

Indira Paudel, Hadas Gerbi, Annat Zisovich, Gal Sapir, Shifra Ben-Dor, Vlad Brumfeld, Tamir Klein

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Water availability is becoming a limiting factor with increasing world population that challenges global food security. Thus, we need to enhance cultivation in increasingly drier and hotter climate and prepare fruit trees for the ongoing climate change. Wild tree species might offer vital information and plant material in face of these challenges.

A year-long comparative field study was conducted to investigate the mechanisms underlying drought tolerance in pear species (cultivated Pyrus communis and Pyrus pyrifolia vs. the wild Pyrus syriaca).

We confirmed the hypothesis of higher drought tolerance in wild pear compared to its cultivated relative. P. syriaca xylem had fewer, narrower vessels, and lower vulnerability to embolism. It showed higher intrinsic water-sue efficiency and more robust seasonal patterns of photosynthesis, hydraulic conductivity, and PIP (plasma intrinsic protein) aquaporin expression. Across species, we identified a ubiquitous gene (PIP1:5/1:6), nine drought-inhibited genes, and two drought-induced genes (PIP1:4 and 2:6/2:7, confirming previous studies).

Our study highlights the potential of using wild relatives of fruit tree species to prepare key crops to a drier and hotter future. The study of PIPS leads the way to a more focused research of the role of these cellular water channels in minimizing tree water loss under drought, while ensuring hydration of specific tissues.

Original language	English
Article number	103832
Number of pages	13
Journal	Environmental and Experimental Botany
Volume	167
Early online date	31 Jul 2019
DOIs	https://doi.org/10.1016/j.envexpbot.2019.103832
State	Published - Nov 2019

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title = "Drought tolerance mechanisms and aquaporin expression of wild vs. cultivated pear tree species in the field",

abstract = "Water availability is becoming a limiting factor with increasing world population that challenges global food security. Thus, we need to enhance cultivation in increasingly drier and hotter climate and prepare fruit trees for the ongoing climate change. Wild tree species might offer vital information and plant material in face of these challenges.A year-long comparative field study was conducted to investigate the mechanisms underlying drought tolerance in pear species (cultivated Pyrus communis and Pyrus pyrifolia vs. the wild Pyrus syriaca).We confirmed the hypothesis of higher drought tolerance in wild pear compared to its cultivated relative. P. syriaca xylem had fewer, narrower vessels, and lower vulnerability to embolism. It showed higher intrinsic water-sue efficiency and more robust seasonal patterns of photosynthesis, hydraulic conductivity, and PIP (plasma intrinsic protein) aquaporin expression. Across species, we identified a ubiquitous gene (PIP1:5/1:6), nine drought-inhibited genes, and two drought-induced genes (PIP1:4 and 2:6/2:7, confirming previous studies).Our study highlights the potential of using wild relatives of fruit tree species to prepare key crops to a drier and hotter future. The study of PIPS leads the way to a more focused research of the role of these cellular water channels in minimizing tree water loss under drought, while ensuring hydration of specific tissues.",

author = "Indira Paudel and Hadas Gerbi and Annat Zisovich and Gal Sapir and Shifra Ben-Dor and Vlad Brumfeld and Tamir Klein",

note = "IP wishes to thank to the Weizmann Prestigious Dean Fellowship program. TK wishes to thank Orna Reisman-Berman of the Ben Gurion University of the Negev for her help in developing the research idea; and Menachem Moshelion of the Hebrew University of Jerusalem and David Granot of the ARO Volcani center for critical reading of an earlier version of the paper. The project was funded in part by the Merle S. Cahn Foundation and the Monroe and Marjorie Burk Fund for Alternative Energy Studies; Mr. and Mrs. Norman Reiser, together with the Weizmann Center for New Scientists; The Yeda-Sela Center for Basic Research; and the Edith & Nathan Goldberg Career Development Chair. The authors declare no conflict of interest. Author contributions IP coordinated the measurements and most of the analysis and provided the first draft; HG helped in the measurements and in writing the manuscript; TK initiated the project, designed the experiment, and wrote the manuscript; AZ and GS helped in coordinating the field sites; SBD defined the aquaporin genes and designed the primers; and VB consulted the microCT scans.",

year = "2019",

month = nov,

doi = "https://doi.org/10.1016/j.envexpbot.2019.103832",

language = "الإنجليزيّة",

volume = "167",

journal = "Environmental and Experimental Botany",

issn = "0098-8472",

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T1 - Drought tolerance mechanisms and aquaporin expression of wild vs. cultivated pear tree species in the field

AU - Paudel, Indira

AU - Gerbi, Hadas

AU - Zisovich, Annat

AU - Sapir, Gal

AU - Ben-Dor, Shifra

AU - Brumfeld, Vlad

AU - Klein, Tamir

N1 - IP wishes to thank to the Weizmann Prestigious Dean Fellowship program. TK wishes to thank Orna Reisman-Berman of the Ben Gurion University of the Negev for her help in developing the research idea; and Menachem Moshelion of the Hebrew University of Jerusalem and David Granot of the ARO Volcani center for critical reading of an earlier version of the paper. The project was funded in part by the Merle S. Cahn Foundation and the Monroe and Marjorie Burk Fund for Alternative Energy Studies; Mr. and Mrs. Norman Reiser, together with the Weizmann Center for New Scientists; The Yeda-Sela Center for Basic Research; and the Edith & Nathan Goldberg Career Development Chair. The authors declare no conflict of interest. Author contributions IP coordinated the measurements and most of the analysis and provided the first draft; HG helped in the measurements and in writing the manuscript; TK initiated the project, designed the experiment, and wrote the manuscript; AZ and GS helped in coordinating the field sites; SBD defined the aquaporin genes and designed the primers; and VB consulted the microCT scans.

PY - 2019/11

Y1 - 2019/11

N2 - Water availability is becoming a limiting factor with increasing world population that challenges global food security. Thus, we need to enhance cultivation in increasingly drier and hotter climate and prepare fruit trees for the ongoing climate change. Wild tree species might offer vital information and plant material in face of these challenges.A year-long comparative field study was conducted to investigate the mechanisms underlying drought tolerance in pear species (cultivated Pyrus communis and Pyrus pyrifolia vs. the wild Pyrus syriaca).We confirmed the hypothesis of higher drought tolerance in wild pear compared to its cultivated relative. P. syriaca xylem had fewer, narrower vessels, and lower vulnerability to embolism. It showed higher intrinsic water-sue efficiency and more robust seasonal patterns of photosynthesis, hydraulic conductivity, and PIP (plasma intrinsic protein) aquaporin expression. Across species, we identified a ubiquitous gene (PIP1:5/1:6), nine drought-inhibited genes, and two drought-induced genes (PIP1:4 and 2:6/2:7, confirming previous studies).Our study highlights the potential of using wild relatives of fruit tree species to prepare key crops to a drier and hotter future. The study of PIPS leads the way to a more focused research of the role of these cellular water channels in minimizing tree water loss under drought, while ensuring hydration of specific tissues.

AB - Water availability is becoming a limiting factor with increasing world population that challenges global food security. Thus, we need to enhance cultivation in increasingly drier and hotter climate and prepare fruit trees for the ongoing climate change. Wild tree species might offer vital information and plant material in face of these challenges.A year-long comparative field study was conducted to investigate the mechanisms underlying drought tolerance in pear species (cultivated Pyrus communis and Pyrus pyrifolia vs. the wild Pyrus syriaca).We confirmed the hypothesis of higher drought tolerance in wild pear compared to its cultivated relative. P. syriaca xylem had fewer, narrower vessels, and lower vulnerability to embolism. It showed higher intrinsic water-sue efficiency and more robust seasonal patterns of photosynthesis, hydraulic conductivity, and PIP (plasma intrinsic protein) aquaporin expression. Across species, we identified a ubiquitous gene (PIP1:5/1:6), nine drought-inhibited genes, and two drought-induced genes (PIP1:4 and 2:6/2:7, confirming previous studies).Our study highlights the potential of using wild relatives of fruit tree species to prepare key crops to a drier and hotter future. The study of PIPS leads the way to a more focused research of the role of these cellular water channels in minimizing tree water loss under drought, while ensuring hydration of specific tissues.

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U2 - https://doi.org/10.1016/j.envexpbot.2019.103832

DO - https://doi.org/10.1016/j.envexpbot.2019.103832

M3 - مقالة

SN - 0098-8472

VL - 167

JO - Environmental and Experimental Botany

JF - Environmental and Experimental Botany

M1 - 103832

ER -

Drought tolerance mechanisms and aquaporin expression of wild vs. cultivated pear tree species in the field

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