DNA G-Quadruplex Is a Transcriptional Control Device That Regulates Memory

Paul R. Marshall; Joshua Davies; Qiongyi Zhao; Wei Siang Liau; Yujin Lee; Dean Basic; Ambika Periyakaruppiah; Esmi L. Zajaczkowski; Laura J. Leighton; Sachithrani U. Madugalle; Mason Musgrove; Marcin Kielar; Arie Maeve Brueckner; Hao Gong; Haobin Ren; Alexander Walsh; Lech Kaczmarczyk; Walker S. Jackson; Alon Chen; Robert C. Spitale; Timothy W. Bredy

doi:10.1523/JNEUROSCI.0093-23.2024

DNA G-Quadruplex Is a Transcriptional Control Device That Regulates Memory

Paul R. Marshall, Joshua Davies, Qiongyi Zhao, Wei Siang Liau, Yujin Lee, Dean Basic, Ambika Periyakaruppiah, Esmi L. Zajaczkowski, Laura J. Leighton, Sachithrani U. Madugalle, Mason Musgrove, Marcin Kielar, Arie Maeve Brueckner, Hao Gong, Haobin Ren, Alexander Walsh, Lech Kaczmarczyk, Walker S. Jackson, Alon Chen, Robert C. SpitaleTimothy W. Bredy

Department of Brain Sciences

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

The conformational state of DNA fine-tunes the transcriptional rate and abundance of RNA. Here, we report that G-quadruplex DNA (G4-DNA) accumulates in neurons, in an experience-dependent manner, and that this is required for the transient silencing and activation of genes that are critically involved in learning and memory in male C57/BL6 mice. In addition, site-specific resolution of G4-DNA by dCas9-mediated deposition of the helicase DHX36 impairs fear extinction memory. Dynamic DNA structure states therefore represent a key molecular mechanism underlying memory consolidation. One-Sentence Summary: G4-DNA is a molecular switch that enables the temporal regulation of the gene expression underlying the formation of fear extinction memory.

Original language	English
Article number	e0093232024
Number of pages	20
Journal	Journal of Neuroscience
Volume	44
Issue number	15
Early online date	28 Feb 2024
DOIs	https://doi.org/10.1523/JNEUROSCI.0093-23.2024
State	Published - 10 Apr 2024

All Science Journal Classification (ASJC) codes

General Neuroscience

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10.1523/JNEUROSCI.0093-23.2024

https://www.biorxiv.org/content/10.1101/2023.01.09.523337v1

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Marshall, P. R., Davies, J., Zhao, Q., Liau, W. S., Lee, Y., Basic, D., Periyakaruppiah, A., Zajaczkowski, E. L., Leighton, L. J., Madugalle, S. U., Musgrove, M., Kielar, M., Brueckner, A. M., Gong, H., Ren, H., Walsh, A., Kaczmarczyk, L., Jackson, W. S., Chen, A., ... Bredy, T. W. (2024). DNA G-Quadruplex Is a Transcriptional Control Device That Regulates Memory. Journal of Neuroscience, 44(15), Article e0093232024. https://doi.org/10.1523/JNEUROSCI.0093-23.2024

@article{8cde947f38d642d787ba59e46aa71b0d,

title = "DNA G-Quadruplex Is a Transcriptional Control Device That Regulates Memory",

abstract = "The conformational state of DNA fine-tunes the transcriptional rate and abundance of RNA. Here, we report that G-quadruplex DNA (G4-DNA) accumulates in neurons, in an experience-dependent manner, and that this is required for the transient silencing and activation of genes that are critically involved in learning and memory in male C57/BL6 mice. In addition, site-specific resolution of G4-DNA by dCas9-mediated deposition of the helicase DHX36 impairs fear extinction memory. Dynamic DNA structure states therefore represent a key molecular mechanism underlying memory consolidation. One-Sentence Summary: G4-DNA is a molecular switch that enables the temporal regulation of the gene expression underlying the formation of fear extinction memory.",

author = "Marshall, {Paul R.} and Joshua Davies and Qiongyi Zhao and Liau, {Wei Siang} and Yujin Lee and Dean Basic and Ambika Periyakaruppiah and Zajaczkowski, {Esmi L.} and Leighton, {Laura J.} and Madugalle, {Sachithrani U.} and Mason Musgrove and Marcin Kielar and Brueckner, {Arie Maeve} and Hao Gong and Haobin Ren and Alexander Walsh and Lech Kaczmarczyk and Jackson, {Walker S.} and Alon Chen and Spitale, {Robert C.} and Bredy, {Timothy W.}",

year = "2024",

month = apr,

day = "10",

doi = "10.1523/JNEUROSCI.0093-23.2024",

language = "الإنجليزيّة",

volume = "44",

journal = "Journal of Neuroscience",

issn = "0270-6474",

publisher = "Society for Neuroscience",

number = "15",

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Marshall, PR, Davies, J, Zhao, Q, Liau, WS, Lee, Y, Basic, D, Periyakaruppiah, A, Zajaczkowski, EL, Leighton, LJ, Madugalle, SU, Musgrove, M, Kielar, M, Brueckner, AM, Gong, H, Ren, H, Walsh, A, Kaczmarczyk, L, Jackson, WS, Chen, A, Spitale, RC & Bredy, TW 2024, 'DNA G-Quadruplex Is a Transcriptional Control Device That Regulates Memory', Journal of Neuroscience, vol. 44, no. 15, e0093232024. https://doi.org/10.1523/JNEUROSCI.0093-23.2024

TY - JOUR

T1 - DNA G-Quadruplex Is a Transcriptional Control Device That Regulates Memory

AU - Marshall, Paul R.

AU - Davies, Joshua

AU - Zhao, Qiongyi

AU - Liau, Wei Siang

AU - Lee, Yujin

AU - Basic, Dean

AU - Periyakaruppiah, Ambika

AU - Zajaczkowski, Esmi L.

AU - Leighton, Laura J.

AU - Madugalle, Sachithrani U.

AU - Musgrove, Mason

AU - Kielar, Marcin

AU - Brueckner, Arie Maeve

AU - Gong, Hao

AU - Ren, Haobin

AU - Walsh, Alexander

AU - Kaczmarczyk, Lech

AU - Jackson, Walker S.

AU - Chen, Alon

AU - Spitale, Robert C.

AU - Bredy, Timothy W.

PY - 2024/4/10

Y1 - 2024/4/10

N2 - The conformational state of DNA fine-tunes the transcriptional rate and abundance of RNA. Here, we report that G-quadruplex DNA (G4-DNA) accumulates in neurons, in an experience-dependent manner, and that this is required for the transient silencing and activation of genes that are critically involved in learning and memory in male C57/BL6 mice. In addition, site-specific resolution of G4-DNA by dCas9-mediated deposition of the helicase DHX36 impairs fear extinction memory. Dynamic DNA structure states therefore represent a key molecular mechanism underlying memory consolidation. One-Sentence Summary: G4-DNA is a molecular switch that enables the temporal regulation of the gene expression underlying the formation of fear extinction memory.

AB - The conformational state of DNA fine-tunes the transcriptional rate and abundance of RNA. Here, we report that G-quadruplex DNA (G4-DNA) accumulates in neurons, in an experience-dependent manner, and that this is required for the transient silencing and activation of genes that are critically involved in learning and memory in male C57/BL6 mice. In addition, site-specific resolution of G4-DNA by dCas9-mediated deposition of the helicase DHX36 impairs fear extinction memory. Dynamic DNA structure states therefore represent a key molecular mechanism underlying memory consolidation. One-Sentence Summary: G4-DNA is a molecular switch that enables the temporal regulation of the gene expression underlying the formation of fear extinction memory.

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U2 - 10.1523/JNEUROSCI.0093-23.2024

DO - 10.1523/JNEUROSCI.0093-23.2024

M3 - مقالة

C2 - 38418220

SN - 0270-6474

VL - 44

JO - Journal of Neuroscience

JF - Journal of Neuroscience

IS - 15

M1 - e0093232024

ER -

DNA G-Quadruplex Is a Transcriptional Control Device That Regulates Memory

Abstract

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