Distinct Single-Cell Morphological Dynamics under Beta-Lactam Antibiotics

Zhizhong Yao, Daniel Kahne, Roy Kishony

Research output: Contribution to journalArticlepeer-review


The bacterial cell wall is conserved in prokaryotes, stabilizing cells against osmotic stress. Beta-lactams inhibit cell-wall synthesis and induce lysis through a bulge-mediated mechanism; however, little is known about the formation dynamics and stability of these bulges. To capture processes of different timescales, we developed an imaging platform combining automated image analysis with live-cell microscopy at high time resolution. Beta-lactam killing of Escherichia coli cells proceeded through four stages: elongation, bulge formation, bulge stagnation, and lysis. Both the cell wall and outer membrane (OM) affect the observed dynamics; damaging the cell wall with different beta-lactams and compromising OM integrity cause different modes and rates of lysis. Our results show that the bulge-formation dynamics are determined by how the cell wall is perturbed. The OM plays an independent role in stabilizing the bulge once it is formed. The stabilized bulge delays lysis and allows recovery upon drug removal.

Original languageEnglish
Pages (from-to)705-712
Number of pages8
JournalMolecular Cell
Issue number5
StatePublished - 14 Dec 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Distinct Single-Cell Morphological Dynamics under Beta-Lactam Antibiotics'. Together they form a unique fingerprint.

Cite this