TY - JOUR
T1 - Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing
AU - Klymiuk, Valentyna
AU - Chawla, Harmeet Singh
AU - Wiebe, Krystalee
AU - Ens, Jennifer
AU - Fatiukha, Andrii
AU - Govta, Liubov
AU - Fahima, Tzion
AU - Pozniak, Curtis J.
N1 - Funding Information: The authors are thankful to Dr. Xianming Chen (USDA) and Dr. Randy Kutcher (University of Saskatchewan) for making stripe rust races available, and to USDA-ARS National Small Grains Collection and Dr. Peng Zhang (University of Sydney) for providing seed material. The authors are grateful to Madison Kist, Xue (Snow) Lin, Lexie Gerl, Ayla Lichtenwald and Maureen Troesch for their technical support. This work was conducted through the 4D Wheat Project (C.J.P.) supported by Genome Canada, Genome Prairie, the Saskatchewan Ministry of Agriculture, Saskatchewan Wheat Development Commission, Alberta Wheat Development Commission, Secan, Agriculture and Agri-Food Canada and the Manitoba Crop Alliance; the “Maximizing Durable Disease Resistance in Wheat” Project (C.J.P.) sponsored by the Saskatchewan Ministry of Agriculture grant number ADF20200353; the Israel Science Foundation, grant number 1366/18 (T.F.). Funding Information: The authors are thankful to Dr. Xianming Chen (USDA) and Dr. Randy Kutcher (University of Saskatchewan) for making stripe rust races available, and to USDA-ARS National Small Grains Collection and Dr. Peng Zhang (University of Sydney) for providing seed material. The authors are grateful to Madison Kist, Xue (Snow) Lin, Lexie Gerl, Ayla Lichtenwald and Maureen Troesch for their technical support. This work was conducted through the 4D Wheat Project (C.J.P.) supported by Genome Canada, Genome Prairie, the Saskatchewan Ministry of Agriculture, Saskatchewan Wheat Development Commission, Alberta Wheat Development Commission, Secan, Agriculture and Agri-Food Canada and the Manitoba Crop Alliance; the “Maximizing Durable Disease Resistance in Wheat” Project (C.J.P.) sponsored by the Saskatchewan Ministry of Agriculture grant number ADF20200353; the Israel Science Foundation, grant number 1366/18 (T.F.). Publisher Copyright: © 2022, The Author(s).
PY - 2022/8/17
Y1 - 2022/8/17
N2 - Durable crop disease resistance is an essential component of global food security. Continuous pathogen evolution leads to a breakdown of resistance and there is a pressing need to characterize new resistance genes for use in plant breeding. Here we identified an accession of wild emmer wheat (Triticum turgidum ssp. dicoccoides), PI 487260, that is highly resistant to multiple stripe rust isolates. Genetic analysis revealed resistance was conferred by a single, incompletely dominant gene designated as Yr84. Through bulked segregant analysis sequencing (BSA-Seq) we identified a 52.7 Mb resistance-associated interval on chromosome 1BS. Detected variants were used to design genetic markers for recombinant screening, further refining the interval of Yr84 to a 2.3–3.3 Mb in tetraploid wheat genomes. This interval contains 34 candidate genes encoding for protein domains involved in disease resistance responses. Furthermore, KASP markers closely-linked to Yr84 were developed to facilitate marker-assisted selection for rust resistance breeding.
AB - Durable crop disease resistance is an essential component of global food security. Continuous pathogen evolution leads to a breakdown of resistance and there is a pressing need to characterize new resistance genes for use in plant breeding. Here we identified an accession of wild emmer wheat (Triticum turgidum ssp. dicoccoides), PI 487260, that is highly resistant to multiple stripe rust isolates. Genetic analysis revealed resistance was conferred by a single, incompletely dominant gene designated as Yr84. Through bulked segregant analysis sequencing (BSA-Seq) we identified a 52.7 Mb resistance-associated interval on chromosome 1BS. Detected variants were used to design genetic markers for recombinant screening, further refining the interval of Yr84 to a 2.3–3.3 Mb in tetraploid wheat genomes. This interval contains 34 candidate genes encoding for protein domains involved in disease resistance responses. Furthermore, KASP markers closely-linked to Yr84 were developed to facilitate marker-assisted selection for rust resistance breeding.
UR - http://www.scopus.com/inward/record.url?scp=85136073091&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/s42003-022-03773-3
DO - https://doi.org/10.1038/s42003-022-03773-3
M3 - Article
C2 - 35978056
SN - 2399-3642
VL - 5
SP - 826
JO - Communications Biology
JF - Communications Biology
IS - 1
M1 - 826
ER -