Direct observation of mammalian cell growth and size regulation

Sungmin Son, Amit Tzur, Yaochung Weng, Paul Jorgensen, Jisoo Kim, Marc W. Kirschner, Scott R. Manalis

Research output: Contribution to journalArticlepeer-review


We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.

Original languageEnglish
Pages (from-to)910-912
Number of pages3
JournalNature Methods
Issue number9
StatePublished - Sep 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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