Direct evidence that late Neanderthal occupation precedes a technological shift in southwestern Italy

Gregorio Oxilia, Eugenio Bortolini, Giulia Marciani, Jessica Cristina Menghi Sartorio, Antonino Vazzana, Matteo Bettuzzi, Daniele Panetta, Simona Arrighi, Federica Badino, Carla Figus, Federico Lugli, Matteo Romandini, Sara Silvestrini, Rita Sorrentino, Adriana Moroni, Carlo Donadio, Maria Pia Morigi, Viviane Slon, Marcello Piperno, Sahra TalamoCarmine Collina, Stefano Benazzi

Research output: Contribution to journalArticlepeer-review


Objectives: During the middle-to-upper Paleolithic transition (50,000 and 40,000 years ago), interaction between Neanderthals and Homo sapiens varied across Europe. In southern Italy, the association between Homo sapiens fossils and non-Mousterian material culture, as well as the mode and tempo of Neanderthal demise, are still vividly debated. In this research, we focus on the study of two human teeth by using 3D geometric morphometric approaches for a reliable taxonomical attribution as well as obtaining new radiometric dates on the archeological sequence. Material and Methods: This work presents two lower deciduous molars uncovered at Roccia San Sebastiano (Mondragone-Caserta, Italy), stratigraphically associated with Mousterian (RSS1) and Uluzzian (RSS2) artifacts. To obtain a probabilistic attribution of the two RSS teeth to each reference taxa group composed of Neanderthals and Homo sapiens, we performed and compared the performance of three supervised learning algorithms (flexible discriminant analysis, multiadaptive regression splines, and random forest) on both crown and cervical outlines obtained by virtual morphometric methods. Results: We show that RSS1, whose Mousterian context appears more recent than 44,800–44,230 cal BP, can be attributed to a Neanderthal, while RSS2, found in an Uluzzian context that we dated to 42,640–42,380 cal BP, is attributed to Homo sapiens. Discussion: This site yields the most recent direct evidence for a Neanderthal presence in southern Italy and confirms a later shift to upper Paleolithic technology in southwestern Italy compared to the earliest Uluzzian evidence at Grotta del Cavallo (Puglia, Italy).

Original languageEnglish
Pages (from-to)18-30
Number of pages13
JournalAmerican Journal of Biological Anthropology
Issue number1
StatePublished - Sep 2022


  • Mediterranean Europe
  • Neanderthal
  • Uluzzian
  • deciduous human molars
  • supervised learning algorithms
  • virtual analysis

All Science Journal Classification (ASJC) codes

  • Anthropology
  • Genetics
  • Medicine(all)
  • Epidemiology
  • Archaeology
  • Palaeontology


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