TY - JOUR
T1 - Dipeptide Nanostructure Assembly and Dynamics via in Situ Liquid-Phase Electron Microscopy
AU - Gnanasekaran, Karthikeyan
AU - Korpanty, Joanna
AU - Berger, Or
AU - Hampu, Nicholas
AU - Halperin-Sternfeld, Michal
AU - Cohen-Gerassi, Dana
AU - Adler-Abramovich, Lihi
AU - Gianneschi, Nathan C.
N1 - Publisher Copyright: © 2021 American Chemical Society.
PY - 2021/10/26
Y1 - 2021/10/26
N2 - In this paper, we report the in situ growth of FF nanotubes examined via liquid-cell transmission electron microscopy (LCTEM). This direct, high spatial, and temporal resolution imaging approach allowed us to observe the growth of peptide-based nanofibrillar structures through directional elongation. Furthermore, the radial growth profile of FF nanotubes through the addition of monomers perpendicular to the tube axis has been observed in real-time with sufficient resolution to directly observe the increase in diameter. Our study demonstrates that the kinetics, dynamics, structure formation, and assembly mechanism of these supramolecular assemblies can be directly monitored using LCTEM. The performance of the peptides and the assemblies they form can be verified and evaluated using post-mortem techniques including time-of-flight secondary ion mass spectrometry (ToF-SIMS).
AB - In this paper, we report the in situ growth of FF nanotubes examined via liquid-cell transmission electron microscopy (LCTEM). This direct, high spatial, and temporal resolution imaging approach allowed us to observe the growth of peptide-based nanofibrillar structures through directional elongation. Furthermore, the radial growth profile of FF nanotubes through the addition of monomers perpendicular to the tube axis has been observed in real-time with sufficient resolution to directly observe the increase in diameter. Our study demonstrates that the kinetics, dynamics, structure formation, and assembly mechanism of these supramolecular assemblies can be directly monitored using LCTEM. The performance of the peptides and the assemblies they form can be verified and evaluated using post-mortem techniques including time-of-flight secondary ion mass spectrometry (ToF-SIMS).
KW - ToF-SIMS
KW - dipeptides
KW - diphenylalanine
KW - liquid-cell TEM
KW - self-assembly
UR - http://www.scopus.com/inward/record.url?scp=85117779414&partnerID=8YFLogxK
U2 - 10.1021/acsnano.1c06130
DO - 10.1021/acsnano.1c06130
M3 - مقالة
C2 - 34623126
SN - 1936-0851
VL - 15
SP - 16542
EP - 16551
JO - ACS Nano
JF - ACS Nano
IS - 10
ER -