TY - JOUR
T1 - Diffusion of small molecule drugs is affected by surface interactions and crowder proteins
AU - Dey, Debabrata
AU - Nunes-Alves, Ariane
AU - Wade, Rebecca C.
AU - Schreiber, Gideon
PY - 2022/10/21
Y1 - 2022/10/21
N2 - Crowded environments are known to affect the diffusion of macromolecules, but their effects on the diffusion of small molecules are largely uncharacterized. We investigate how three protein crowders, bovine serum albumin (BSA), hen egg -white lysozyme, and myoglobin, influence the diffusion rates and interactions of four small molecules: fluorescein, and three drugs, doxorubicin, glycogen syn-thase kinase-3 inhibitor SB216763, and quinacrine. Using Line-FRAP measure-ments, Brownian dynamics simulations, and molecular docking, we find that the diffusion rates of the small molecules are highly affected by self-aggregation, in-teractions with the proteins, and surface adsorption. The diffusion of fluorescein is decreased because of its interactions with the protein crowders and their sur-face adsorption. Protein crowders increase the diffusion rates of doxorubicin and SB216763 by reducing surface interactions and self-aggregation, respectively. Quinacrine diffusion was not affected by protein crowders. The mechanistic in-sights gained here may assist in optimization of compounds for higher mobility in complex macromolecular environments.
AB - Crowded environments are known to affect the diffusion of macromolecules, but their effects on the diffusion of small molecules are largely uncharacterized. We investigate how three protein crowders, bovine serum albumin (BSA), hen egg -white lysozyme, and myoglobin, influence the diffusion rates and interactions of four small molecules: fluorescein, and three drugs, doxorubicin, glycogen syn-thase kinase-3 inhibitor SB216763, and quinacrine. Using Line-FRAP measure-ments, Brownian dynamics simulations, and molecular docking, we find that the diffusion rates of the small molecules are highly affected by self-aggregation, in-teractions with the proteins, and surface adsorption. The diffusion of fluorescein is decreased because of its interactions with the protein crowders and their sur-face adsorption. Protein crowders increase the diffusion rates of doxorubicin and SB216763 by reducing surface interactions and self-aggregation, respectively. Quinacrine diffusion was not affected by protein crowders. The mechanistic in-sights gained here may assist in optimization of compounds for higher mobility in complex macromolecular environments.
U2 - https://doi.org/10.1016/j.isci.2022.105088
DO - https://doi.org/10.1016/j.isci.2022.105088
M3 - مقالة
C2 - 36157590
SN - 2589-0042
VL - 25
JO - iScience
JF - iScience
IS - 10
M1 - 105088
ER -