@article{ab68760152694f61acbf8b6c27a88e8a,
title = "Differential translation tunes uneven production of operon-encoded proteins",
abstract = "Clustering of functionally related genes in operons allows for coregulated gene expression in prokaryotes. This is advantageous when equal amounts of gene products are required. Production of protein complexes with an uneven stoichiometry, however, requires tuning mechanisms to generate subunits in appropriate relative quantities. Using comparative genomic analysis, we show that differential translation is a key determinant of modulated expression of genes clustered in operons and that codon bias generally is the best insilico indicator of unequal protein production. Variable ribosome density profiles of polycistronic transcripts correlate strongly with differential translation patterns. In addition, we provide experimental evidence that de novo initiation of translation can occur at intercistronic sites, allowing for differential translation of any gene irrespective of its position on a polycistronic messenger. Thus, modulation of translation efficiency appears to be auniversal mode of control in bacteria and archaea that allows for differential production of operon-encodedproteins",
author = "Quax, {Tessa E. F.} and Wolf, {Yuri I.} and Koehorst, {Jasper J.} and Omri Wurtzel and {van der Oost}, {der Oost, Richard} and Wenqi Ran and Fabian Blombach and Makarova, {Kira S.} and Brouns, {Stan J. J.} and Forster, {Anthony C.} and Wagner, {E. Gerhart H.} and Rotem Sorek and Koonin, {Eugene V.} and {van der Oost}, {der Oost, John} and Richard vanderOost and John vanderOost",
note = "Netherlands Organization for Scientific Research (NWO) via an ALW TOP grant [854.10.003]; ALW Vidi grant [864.11.005]; Ministere de l'Enseignement Superieur et de la Recherche de France; U.S. Department of Health and Human Services intramural program (National Library of Medicine, NIH); Azrieli PhD fellowship; ERC-StG grant [260432]; Israeli Science Foundation [ISF-1303/12]; Swedish Research Council; Uppsala RNA Research CentreWe thank Jonathan Weissman and Gene-Wei Li (UCSF) for providing ribosome profiling data and stimulating discussions. This project was funded by the Netherlands Organization for Scientific Research (NWO) via an ALW TOP grant (854.10.003) to J.v.d.O. and an ALW Vidi grant (864.11.005) to S.J.J.B. T. E. F. Q. was supported by a fellowship from the Ministere de l'Enseignement Superieur et de la Recherche de France. Y.I.W., W. R., K. S. M., and E. V. K. were supported by the U.S. Department of Health and Human Services intramural program (National Library of Medicine, NIH). O.W. was supported by an Azrieli PhD fellowship, and R. S. was supported by an ERC-StG grant (260432) and the Israeli Science Foundation (grant ISF-1303/12). A. C. F. and E. G. H. W. were supported by grants from the Swedish Research Council, including the Uppsala RNA Research Centre.",
year = "2013",
month = may,
day = "12",
doi = "https://doi.org/10.1016/j.celrep.2013.07.049",
language = "الإنجليزيّة",
volume = "4",
pages = "938--944",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",
}