Diazirine based photoaffinity labeling

Luba Dubinsky; Bastiaan P. Krom; Michael M. Meijler

doi:10.1016/j.bmc.2011.06.066

Diazirine based photoaffinity labeling

Luba Dubinsky, Bastiaan P. Krom, Michael M. Meijler

Department of Chemistry

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.

Original language	American English
Pages (from-to)	554-570
Number of pages	17
Journal	Bioorganic and Medicinal Chemistry
Volume	20
Issue number	2
DOIs	https://doi.org/10.1016/j.bmc.2011.06.066
State	Published - 15 Jan 2012

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Chemical proteomics
Diazirine
Photoaffinity labeling
Photochemistry
Protein profiling

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmc.2011.06.066

Cite this

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title = "Diazirine based photoaffinity labeling",

abstract = "Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.",

keywords = "Chemical proteomics, Diazirine, Photoaffinity labeling, Photochemistry, Protein profiling",

author = "Luba Dubinsky and Krom, \{Bastiaan P.\} and Meijler, \{Michael M.\}",

year = "2012",

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doi = "10.1016/j.bmc.2011.06.066",

language = "American English",

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journal = "Bioorganic and Medicinal Chemistry",

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AU - Dubinsky, Luba

AU - Krom, Bastiaan P.

AU - Meijler, Michael M.

PY - 2012/1/15

Y1 - 2012/1/15

N2 - Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.

AB - Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.

KW - Chemical proteomics

KW - Diazirine

KW - Photoaffinity labeling

KW - Photochemistry

KW - Protein profiling

UR - http://www.scopus.com/inward/record.url?scp=84855816511&partnerID=8YFLogxK

U2 - 10.1016/j.bmc.2011.06.066

DO - 10.1016/j.bmc.2011.06.066

M3 - Article

SN - 0968-0896

VL - 20

SP - 554

EP - 570

JO - Bioorganic and Medicinal Chemistry

JF - Bioorganic and Medicinal Chemistry

IS - 2

ER -

Diazirine based photoaffinity labeling

Abstract

UN SDGs

Keywords

All Science Journal Classification (ASJC) codes

Access to Document

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