Development of migrating tendon-bone attachments involves replacement of progenitor populations

Tendon-bone attachment sites, called entheses, are essential for musculoskeletal function. They are formed embryonically by Sox9+ progenitors and continue to develop postnatally, utilizing Gil1 lineage cells. Despite their importance, we lack information on the transition from embryonic to mature enthesis and on the relation between Sox9+ progenitors and the Gil1 lineage. Here, by performing a series of lineage tracing experiments in mice, we identify the onset of Gil1 lineage contribution to different entheses. We show that Oil expression is regulated embryonically by SHH signaling, whereas postnatally it is maintained by IHH signaling. During bone elongation, some entheses migrate along the bone shaft, whereas others remain stationary. Interestingly, in stationary entheses Sox9(+) cells differentiate into the Gil1 lineage, but in migrating entheses this lineage is replaced by Gil1 lineage. These Gli1(+) progenitors are defined embryonically to occupy the different domains of the mature enthesis. Overall, these findings demonstrate a developmental strategy whereby one progenitor population establishes a simple embryonic tissue, whereas another population contributes to its maturation. Moreover, they suggest that different cell populations may be considered for cell-based therapy of enthesis injuries.