Development and evaluation of a cucumber TILLING population

Rina Fraenkel; Irina Kovalski; Christelle Troadec; Abdelhafid Bendahmane; Rafael Perl-Treves

doi:https://doi.org/10.1186/1756-0500-7-846

Development and evaluation of a cucumber TILLING population

Rina Fraenkel, Irina Kovalski, Christelle Troadec, Abdelhafid Bendahmane, Rafael Perl-Treves

The Mina and Everard Goodman Faculty of Life Sciences

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Ordered collections of mutants serve as invaluable tools in biological research. TILLING (Targeting Induced Local Lesions IN Genomes) provides an efficient method to discover, in mutagenized populations, the possible phenotypes controlled by gene sequences whose function is unknown. This method can replace transgenic techniques for the functional validation of cloned genes, especially in the case of transformation-recalcitrant plants such as cucumber. Results: We report the development of a TILLING cucumber population, generated by EMS mutagenesis in the Poinsett76 genetic background. The population was evaluated by screening for morphological mutations, and a range of developmental, pigmentation and spontaneous lesion mutants were observed. Suitability for detecting single nucleotide polymorphism in selected genes has been tested by screening a sample of amplicons, with detection rate of 1 SNP in ∼1 Mbp. Conclusion: The population described in this Research Note represents a useful asset in cucumber research, to be exploited for forward genetic screens and functional genomics purposes.

Original language	English
Article number	846
Journal	BMC Research Notes
Volume	7
Issue number	1
DOIs	https://doi.org/10.1186/1756-0500-7-846
State	Published - 26 Nov 2014

Keywords

Cucumis sativus
EMS mutagenesis
Mutant screen
TILLING

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology

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https://doi.org/10.1186/1756-0500-7-846

Cite this

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title = "Development and evaluation of a cucumber TILLING population",

abstract = "Background: Ordered collections of mutants serve as invaluable tools in biological research. TILLING (Targeting Induced Local Lesions IN Genomes) provides an efficient method to discover, in mutagenized populations, the possible phenotypes controlled by gene sequences whose function is unknown. This method can replace transgenic techniques for the functional validation of cloned genes, especially in the case of transformation-recalcitrant plants such as cucumber. Results: We report the development of a TILLING cucumber population, generated by EMS mutagenesis in the Poinsett76 genetic background. The population was evaluated by screening for morphological mutations, and a range of developmental, pigmentation and spontaneous lesion mutants were observed. Suitability for detecting single nucleotide polymorphism in selected genes has been tested by screening a sample of amplicons, with detection rate of 1 SNP in ∼1 Mbp. Conclusion: The population described in this Research Note represents a useful asset in cucumber research, to be exploited for forward genetic screens and functional genomics purposes.",

keywords = "Cucumis sativus, EMS mutagenesis, Mutant screen, TILLING",

author = "Rina Fraenkel and Irina Kovalski and Christelle Troadec and Abdelhafid Bendahmane and Rafael Perl-Treves",

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doi = "https://doi.org/10.1186/1756-0500-7-846",

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T1 - Development and evaluation of a cucumber TILLING population

AU - Fraenkel, Rina

AU - Kovalski, Irina

AU - Troadec, Christelle

AU - Bendahmane, Abdelhafid

AU - Perl-Treves, Rafael

PY - 2014/11/26

Y1 - 2014/11/26

N2 - Background: Ordered collections of mutants serve as invaluable tools in biological research. TILLING (Targeting Induced Local Lesions IN Genomes) provides an efficient method to discover, in mutagenized populations, the possible phenotypes controlled by gene sequences whose function is unknown. This method can replace transgenic techniques for the functional validation of cloned genes, especially in the case of transformation-recalcitrant plants such as cucumber. Results: We report the development of a TILLING cucumber population, generated by EMS mutagenesis in the Poinsett76 genetic background. The population was evaluated by screening for morphological mutations, and a range of developmental, pigmentation and spontaneous lesion mutants were observed. Suitability for detecting single nucleotide polymorphism in selected genes has been tested by screening a sample of amplicons, with detection rate of 1 SNP in ∼1 Mbp. Conclusion: The population described in this Research Note represents a useful asset in cucumber research, to be exploited for forward genetic screens and functional genomics purposes.

AB - Background: Ordered collections of mutants serve as invaluable tools in biological research. TILLING (Targeting Induced Local Lesions IN Genomes) provides an efficient method to discover, in mutagenized populations, the possible phenotypes controlled by gene sequences whose function is unknown. This method can replace transgenic techniques for the functional validation of cloned genes, especially in the case of transformation-recalcitrant plants such as cucumber. Results: We report the development of a TILLING cucumber population, generated by EMS mutagenesis in the Poinsett76 genetic background. The population was evaluated by screening for morphological mutations, and a range of developmental, pigmentation and spontaneous lesion mutants were observed. Suitability for detecting single nucleotide polymorphism in selected genes has been tested by screening a sample of amplicons, with detection rate of 1 SNP in ∼1 Mbp. Conclusion: The population described in this Research Note represents a useful asset in cucumber research, to be exploited for forward genetic screens and functional genomics purposes.

KW - Cucumis sativus

KW - EMS mutagenesis

KW - Mutant screen

KW - TILLING

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M3 - مقالة

SN - 1756-0500

VL - 7

JO - BMC Research Notes

JF - BMC Research Notes

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M1 - 846

ER -

Development and evaluation of a cucumber TILLING population

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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