Abstract
Fluorescence is a type of electromagnetic spectroscopy that employs emission from a sample. It involves using a beam of light that excites the electrons in specific compounds and causes them to emit light of a lower energy. As proof of principle, a computer controlled experiment is described employing a single microliter capillary tube setup, using a two wavelength excitation of a single wavelength emission of hydroxypyrene trisulfonate. The same setup can be manipulated for the other pH-sensitive fluorescence ratiometric systems. In addition, this setup can be scaled up for high-throughput measurements.
| Original language | English |
|---|---|
| Pages (from-to) | 627-634 |
| Number of pages | 8 |
| Journal | Instrumentation Science and Technology |
| Volume | 42 |
| Issue number | 6 |
| DOIs | |
| State | Published - 15 Nov 2014 |
Keywords
- Capillaries
- Fluorescence
- Spectroscopy
ASJC Scopus subject areas
- General Chemical Engineering
- Instrumentation
- General Environmental Science
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