Detection and characterization of ubiquitylated H2B in mammalian cells

Efrat Shema, Moshe Oren, Neri Minsky

Research output: Contribution to journalArticlepeer-review

Abstract

Histone H2B ubiquitylation was shown to be associated with actively transcribed genes in mammalian cells and has been suggested to be involved in transcriptional regulation. Despite the limited applicability of genetic tools to analyze H2B ubiquitylation in mammals, several biochemical and immunological approaches have been successfully implemented to study this modification. Here we describe several techniques to detect ubiquitylated H2B in mammalian cells and to dissect its genomic localization.

Original languageEnglish
Pages (from-to)326-330
Number of pages5
JournalMethods
Volume54
Issue number3
DOIs
StatePublished - Jul 2011

All Science Journal Classification (ASJC) codes

  • General Biochemistry,Genetics and Molecular Biology
  • Molecular Biology

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