Detection and characterization of ubiquitylated H2B in mammalian cells

Efrat Shema; Moshe Oren; Neri Minsky

doi:10.1016/j.ymeth.2011.03.005

Detection and characterization of ubiquitylated H2B in mammalian cells

Efrat Shema, Moshe Oren, Neri Minsky

Department of Molecular Cell Biology

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Histone H2B ubiquitylation was shown to be associated with actively transcribed genes in mammalian cells and has been suggested to be involved in transcriptional regulation. Despite the limited applicability of genetic tools to analyze H2B ubiquitylation in mammals, several biochemical and immunological approaches have been successfully implemented to study this modification. Here we describe several techniques to detect ubiquitylated H2B in mammalian cells and to dissect its genomic localization.

Original language	English
Pages (from-to)	326-330
Number of pages	5
Journal	Methods
Volume	54
Issue number	3
DOIs	https://doi.org/10.1016/j.ymeth.2011.03.005
State	Published - Jul 2011

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology
Molecular Biology

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10.1016/j.ymeth.2011.03.005

Cite this

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title = "Detection and characterization of ubiquitylated H2B in mammalian cells",

abstract = "Histone H2B ubiquitylation was shown to be associated with actively transcribed genes in mammalian cells and has been suggested to be involved in transcriptional regulation. Despite the limited applicability of genetic tools to analyze H2B ubiquitylation in mammals, several biochemical and immunological approaches have been successfully implemented to study this modification. Here we describe several techniques to detect ubiquitylated H2B in mammalian cells and to dissect its genomic localization.",

author = "Efrat Shema and Moshe Oren and Neri Minsky",

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AB - Histone H2B ubiquitylation was shown to be associated with actively transcribed genes in mammalian cells and has been suggested to be involved in transcriptional regulation. Despite the limited applicability of genetic tools to analyze H2B ubiquitylation in mammals, several biochemical and immunological approaches have been successfully implemented to study this modification. Here we describe several techniques to detect ubiquitylated H2B in mammalian cells and to dissect its genomic localization.

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DO - 10.1016/j.ymeth.2011.03.005

M3 - مقالة

SN - 1046-2023

VL - 54

SP - 326

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JO - Methods

JF - Methods

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Detection and characterization of ubiquitylated H2B in mammalian cells

Abstract

All Science Journal Classification (ASJC) codes

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