@inproceedings{8455555f8c714aa0ab7ebad3ef19868c,
title = "Designing a quantifiable detection method for the optimization of gold nanoparticle based gene therapy",
abstract = "Gold nanoparticles (GNPs) are becoming an increasingly prominent biomedical tool. GNPs are biocompatible and can carry high payloads and a wide array of biological materials, making them an ideal delivery vector for various therapeutics, such as gene therapy. However, one major obstacle to clinical application is endosomal entrapment and subsequent degradation of the nanoparticle-therapy complex. Coating GNPs with an endosomal escape agent can serve as an effective approach to overcome this challenge. This study explores the probability of different types of coated GNPs to perform endosomal escape. We used a novel, multi-modal approach applying fluorescent confocal microscopy, as well as sophisticated image analysis, to provide a quantitative and uniform method that can denote endosomal escape efficacy. Our findings can ultimately advance understanding of endosomal escape abilities of various GNP coatings and promote their application for gene therapy.",
keywords = "Gold nanoparticles, cationic polymers, endosomal escape",
author = "Jacqueline Labovitz and Menachem Motiei and Tamar Sadan and Dror Fixler and Rachela Popovtzer",
note = "Publisher Copyright: Copyright {\textcopyright} 2022 SPIE.; Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX 2022 ; Conference date: 20-02-2022 Through 24-02-2022",
year = "2022",
doi = "https://doi.org/10.1117/12.2608944",
language = "الإنجليزيّة",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Dror Fixler and Goldys, {Ewa M.} and Sebastian Wachsmann-Hogiu",
booktitle = "Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX",
address = "الولايات المتّحدة",
}