TY - JOUR
T1 - Decorating Bacteria with Self-Assembled Synthetic Receptors
AU - Lahav-Mankovski, Naama
AU - Prasad, Pragati Kishore
AU - Oppenheimer-Low, Noa
AU - Raviv, Gal
AU - Dadosh, Tali
AU - Unger, Tamar
AU - Salame, Tomer Meir
AU - Motiei, Leila
AU - Margulies, David
N1 - This research was supported by the Israel Science Foundation (ISF). We thank Prof. Ronit Satchi-Fainaro from Tel Aviv University for providing the KB-cells. We thank Dr. Shira Albeck (Life Sciences Core Facilities, Weizmann Institute of Science) for her assistance in developing the His-tag anchor. Author contributions D.M. initiated the research idea. N.L-M., P.K.P., and L.M. performed the experiments and analyzed the data. N.O.-L. synthesized one of the Cy5-ODN-1 strands. G.R. performed the experiment with Cy5-ODN-1 and EDTA. T.U. conducted the cloning and expression of His-OmpC in bacteria. N.L.-M. and T.D. performed the super-resolution imaging. N.L.-M., P.K.P., and T.M.S. performed and analyzed the FACS experiments. T.D. assisted in designing the experiment involving the adhesion of bacteria to gold surfaces. N.L.-M., L.M., and D.M. wrote the manuscript. All authors read and commented on the manuscript.
PY - 2020/3/10
Y1 - 2020/3/10
N2 - The responses of cells to their surroundings are mediated by the binding of cell surface proteins (CSPs) to extracellular signals. Such processes are regulated via dynamic changes in the structure, composition, and expression levels of CSPs. In this study, we demonstrate the possibility of decorating bacteria with artificial, self-assembled receptors that imitate the dynamic features of CSPs. We show that the local concentration of these receptors on the bacterial membrane and their structure can be reversibly controlled using suitable chemical signals, in a way that resembles changes that occur with CSP expression levels or posttranslational modifications (PTMs), respectively. We also show that these modifications can endow the bacteria with programmable properties, akin to the way CSP responses can induce cellular functions. By programming the bacteria to glow, adhere to surfaces, or interact with proteins or mammalian cells, we demonstrate the potential to tailor such biomimetic systems for specific applications.
AB - The responses of cells to their surroundings are mediated by the binding of cell surface proteins (CSPs) to extracellular signals. Such processes are regulated via dynamic changes in the structure, composition, and expression levels of CSPs. In this study, we demonstrate the possibility of decorating bacteria with artificial, self-assembled receptors that imitate the dynamic features of CSPs. We show that the local concentration of these receptors on the bacterial membrane and their structure can be reversibly controlled using suitable chemical signals, in a way that resembles changes that occur with CSP expression levels or posttranslational modifications (PTMs), respectively. We also show that these modifications can endow the bacteria with programmable properties, akin to the way CSP responses can induce cellular functions. By programming the bacteria to glow, adhere to surfaces, or interact with proteins or mammalian cells, we demonstrate the potential to tailor such biomimetic systems for specific applications.
UR - http://www.scopus.com/inward/record.url?scp=85081628878&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-14336-7
DO - 10.1038/s41467-020-14336-7
M3 - مقالة
C2 - 32157077
SN - 2041-1723
VL - 11
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 1299
ER -