Decoding Human Cytomegalovirus

Noam Stern-Ginossar; Ben Weisburd; Annette Michalski; Vu Thuy Khanh Vu Thuy Khanh Le; Marco Y. Hein; Sheng-Xiong Huang; Ming Ma; Ben Shen; Shu-Bing Qian; Hartmut Hengel; Matthias Mann; Nicholas T. Ingolia; Jonathan S. Weissman

doi:https://doi.org/10.1126/science.1227919

Decoding Human Cytomegalovirus

Noam Stern-Ginossar, Ben Weisburd, Annette Michalski, Vu Thuy Khanh Vu Thuy Khanh Le, Marco Y. Hein, Sheng-Xiong Huang, Ming Ma, Ben Shen, Shu-Bing Qian, Hartmut Hengel, Matthias Mann, Nicholas T. Ingolia, Jonathan S. Weissman

Department of Molecular Genetics

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

The human cytomegalovirus (HCMV) genome was sequenced 20 years ago. However, like those of other complex viruses, our understanding of its protein coding potential is far from complete. We used ribosome profiling and transcript analysis to experimentally define the HCMV translation products and follow their temporal expression. We identified hundreds of previously unidentified open reading frames and confirmed a fraction by means of mass spectrometry. We found that regulated use of alternative transcript start sites plays a broad role in enabling tight temporal control of HCMV protein expression and allowing multiple distinct polypeptides to be generated from a single genomic locus. Our results reveal an unanticipated complexity to the HCMV coding capacity and illustrate the role of regulated changes in transcript start sites in generating this complexity.

Original language	English
Pages (from-to)	1088-1093
Number of pages	6
Journal	Science
Volume	338
Issue number	6110
DOIs	https://doi.org/10.1126/science.1227919
State	Published - 23 Nov 2012

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title = "Decoding Human Cytomegalovirus",

abstract = "The human cytomegalovirus (HCMV) genome was sequenced 20 years ago. However, like those of other complex viruses, our understanding of its protein coding potential is far from complete. We used ribosome profiling and transcript analysis to experimentally define the HCMV translation products and follow their temporal expression. We identified hundreds of previously unidentified open reading frames and confirmed a fraction by means of mass spectrometry. We found that regulated use of alternative transcript start sites plays a broad role in enabling tight temporal control of HCMV protein expression and allowing multiple distinct polypeptides to be generated from a single genomic locus. Our results reveal an unanticipated complexity to the HCMV coding capacity and illustrate the role of regulated changes in transcript start sites in generating this complexity.",

author = "Noam Stern-Ginossar and Ben Weisburd and Annette Michalski and {Vu Thuy Khanh Le}, {Vu Thuy Khanh} and Hein, {Marco Y.} and Sheng-Xiong Huang and Ming Ma and Ben Shen and Shu-Bing Qian and Hartmut Hengel and Matthias Mann and Ingolia, {Nicholas T.} and Weissman, {Jonathan S.}",

year = "2012",

month = nov,

day = "23",

doi = "https://doi.org/10.1126/science.1227919",

language = "الإنجليزيّة",

volume = "338",

pages = "1088--1093",

journal = "Science",

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publisher = "American Association for the Advancement of Science",

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TY - JOUR

T1 - Decoding Human Cytomegalovirus

AU - Stern-Ginossar, Noam

AU - Weisburd, Ben

AU - Michalski, Annette

AU - Vu Thuy Khanh Le, Vu Thuy Khanh

AU - Hein, Marco Y.

AU - Huang, Sheng-Xiong

AU - Ma, Ming

AU - Shen, Ben

AU - Qian, Shu-Bing

AU - Hengel, Hartmut

AU - Mann, Matthias

AU - Ingolia, Nicholas T.

AU - Weissman, Jonathan S.

PY - 2012/11/23

Y1 - 2012/11/23

N2 - The human cytomegalovirus (HCMV) genome was sequenced 20 years ago. However, like those of other complex viruses, our understanding of its protein coding potential is far from complete. We used ribosome profiling and transcript analysis to experimentally define the HCMV translation products and follow their temporal expression. We identified hundreds of previously unidentified open reading frames and confirmed a fraction by means of mass spectrometry. We found that regulated use of alternative transcript start sites plays a broad role in enabling tight temporal control of HCMV protein expression and allowing multiple distinct polypeptides to be generated from a single genomic locus. Our results reveal an unanticipated complexity to the HCMV coding capacity and illustrate the role of regulated changes in transcript start sites in generating this complexity.

AB - The human cytomegalovirus (HCMV) genome was sequenced 20 years ago. However, like those of other complex viruses, our understanding of its protein coding potential is far from complete. We used ribosome profiling and transcript analysis to experimentally define the HCMV translation products and follow their temporal expression. We identified hundreds of previously unidentified open reading frames and confirmed a fraction by means of mass spectrometry. We found that regulated use of alternative transcript start sites plays a broad role in enabling tight temporal control of HCMV protein expression and allowing multiple distinct polypeptides to be generated from a single genomic locus. Our results reveal an unanticipated complexity to the HCMV coding capacity and illustrate the role of regulated changes in transcript start sites in generating this complexity.

U2 - https://doi.org/10.1126/science.1227919

DO - https://doi.org/10.1126/science.1227919

M3 - مقالة

SN - 0036-8075

VL - 338

SP - 1088

EP - 1093

JO - Science

JF - Science

IS - 6110

ER -

Decoding Human Cytomegalovirus

Abstract

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