DARPin_9-29-Targeted Mini Gold Nanorods Specifically Eliminate HER2-Overexpressing Cancer Cells

Galina Proshkina; Sergey Deyev; Anastasiya Ryabova; Francesco Tavanti; Maria Cristina Menziani; Roy Cohen; Liat Katrivas; Alexander Kotlyar

doi:10.1021/acsami.9b10441

DARPin_9-29-Targeted Mini Gold Nanorods Specifically Eliminate HER2-Overexpressing Cancer Cells

Galina Proshkina, Sergey Deyev, Anastasiya Ryabova, Francesco Tavanti, Maria Cristina Menziani, Roy Cohen, Liat Katrivas, Alexander Kotlyar

Biochemistry Molecular Biology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

We have demonstrated that designed ankyrin repeat protein (DARPin)_9-29, which specifically targets human epidermal growth factor receptor 2 (HER2), binds tightly to gold mini nanorods (GNRs). Molecular dynamic simulations showed that a single layer of DARPin_9-29 molecules is formed on the surface of the nanorod and that conjugation with the nanorod does not involve the protein's domain responsible for specific binding to HER2. The nanorod-DARPin (DARPin-GNR) conjugate is specifically bound (in nanomolar concentrations) to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2. Illumination by near-infrared light (850 nm) led to almost complete eradication of the conjugate-treated SK-BR-3 cells; the viability of epithelial human breast cancer cells expressing normal amounts of the receptor was much less affected by the illumination. The results reported here pave the way toward application of DARPin-GNR conjugates in phototherapy of cancer.

Original language	English
Pages (from-to)	34645-34651
Number of pages	7
Journal	ACS Applied Materials and Interfaces
Volume	11
Issue number	38
DOIs	https://doi.org/10.1021/acsami.9b10441
State	Published - 25 Sep 2019

Keywords

DARPin
gold nanorods
molecular dynamic simulations
near-infrared illumination
tumor cells

All Science Journal Classification (ASJC) codes

General Materials Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acsami.9b10441

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title = "DARPin_9-29-Targeted Mini Gold Nanorods Specifically Eliminate HER2-Overexpressing Cancer Cells",

abstract = "We have demonstrated that designed ankyrin repeat protein (DARPin)_9-29, which specifically targets human epidermal growth factor receptor 2 (HER2), binds tightly to gold mini nanorods (GNRs). Molecular dynamic simulations showed that a single layer of DARPin_9-29 molecules is formed on the surface of the nanorod and that conjugation with the nanorod does not involve the protein's domain responsible for specific binding to HER2. The nanorod-DARPin (DARPin-GNR) conjugate is specifically bound (in nanomolar concentrations) to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2. Illumination by near-infrared light (850 nm) led to almost complete eradication of the conjugate-treated SK-BR-3 cells; the viability of epithelial human breast cancer cells expressing normal amounts of the receptor was much less affected by the illumination. The results reported here pave the way toward application of DARPin-GNR conjugates in phototherapy of cancer.",

keywords = "DARPin, gold nanorods, molecular dynamic simulations, near-infrared illumination, tumor cells",

author = "Galina Proshkina and Sergey Deyev and Anastasiya Ryabova and Francesco Tavanti and Menziani, {Maria Cristina} and Roy Cohen and Liat Katrivas and Alexander Kotlyar",

note = "Publisher Copyright: Copyright {\textcopyright} 2019 American Chemical Society.",

year = "2019",

month = sep,

day = "25",

doi = "10.1021/acsami.9b10441",

language = "الإنجليزيّة",

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T1 - DARPin_9-29-Targeted Mini Gold Nanorods Specifically Eliminate HER2-Overexpressing Cancer Cells

AU - Proshkina, Galina

AU - Deyev, Sergey

AU - Ryabova, Anastasiya

AU - Tavanti, Francesco

AU - Menziani, Maria Cristina

AU - Cohen, Roy

AU - Katrivas, Liat

AU - Kotlyar, Alexander

PY - 2019/9/25

Y1 - 2019/9/25

N2 - We have demonstrated that designed ankyrin repeat protein (DARPin)_9-29, which specifically targets human epidermal growth factor receptor 2 (HER2), binds tightly to gold mini nanorods (GNRs). Molecular dynamic simulations showed that a single layer of DARPin_9-29 molecules is formed on the surface of the nanorod and that conjugation with the nanorod does not involve the protein's domain responsible for specific binding to HER2. The nanorod-DARPin (DARPin-GNR) conjugate is specifically bound (in nanomolar concentrations) to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2. Illumination by near-infrared light (850 nm) led to almost complete eradication of the conjugate-treated SK-BR-3 cells; the viability of epithelial human breast cancer cells expressing normal amounts of the receptor was much less affected by the illumination. The results reported here pave the way toward application of DARPin-GNR conjugates in phototherapy of cancer.

AB - We have demonstrated that designed ankyrin repeat protein (DARPin)_9-29, which specifically targets human epidermal growth factor receptor 2 (HER2), binds tightly to gold mini nanorods (GNRs). Molecular dynamic simulations showed that a single layer of DARPin_9-29 molecules is formed on the surface of the nanorod and that conjugation with the nanorod does not involve the protein's domain responsible for specific binding to HER2. The nanorod-DARPin (DARPin-GNR) conjugate is specifically bound (in nanomolar concentrations) to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2. Illumination by near-infrared light (850 nm) led to almost complete eradication of the conjugate-treated SK-BR-3 cells; the viability of epithelial human breast cancer cells expressing normal amounts of the receptor was much less affected by the illumination. The results reported here pave the way toward application of DARPin-GNR conjugates in phototherapy of cancer.

KW - DARPin

KW - gold nanorods

KW - molecular dynamic simulations

KW - near-infrared illumination

KW - tumor cells

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U2 - 10.1021/acsami.9b10441

DO - 10.1021/acsami.9b10441

M3 - مقالة

SN - 1944-8244

VL - 11

SP - 34645

EP - 34651

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 38

ER -

DARPin_9-29-Targeted Mini Gold Nanorods Specifically Eliminate HER2-Overexpressing Cancer Cells

Abstract

Keywords

All Science Journal Classification (ASJC) codes

UN SDGs

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