TY - JOUR
T1 - Crystallization and preliminary X-ray crystallographic analysis of the catalytic domain of membrane type 1 matrix metalloproteinase
AU - Ogata, H
AU - Decaneto, E
AU - Grossman, Moran
AU - Havenith, M
AU - Sagi, Irit
AU - Lubitz, W
AU - Knipp, M
N1 - Cluster of Excellence RESOLV [EXC 1069]; Deutsche Forschungsgemeinschaft; Max Planck Society We are grateful for the technical assistance of Inge Heise, Norbert Dickmann and Yvonne Brandenburger (MPI for Chemical Energy Conversion, Mulheim, Germany). We thank the staff of beamline BL14.2 at BESSY II (Helmholtz-Zentrum Berlin, Germany) for their assistance during data collection. This work was supported by the Cluster of Excellence RESOLV (EXC 1069) funded by the Deutsche Forschungsgemeinschaft. Financial support was also obtained from the Max Planck Society.
PY - 2014/2
Y1 - 2014/2
N2 - Membrane type 1 matrix metalloproteinase (MT1-MMP) belongs to the large family of zinc-dependent endopeptidases termed MMPs that are located in the extracellular matrix. MT1-MMP was crystallized at 277 K using the vapour-diffusion method with PEG as a precipitating agent. Data sets for MT1-MMP were collected to 2.24 Å resolution at 100 K. The crystals belonged to space group P43212, with unit-cell parameters a = 62.99, c = 122.60 Å. The crystal contained one molecule per asymmetric unit, with a Matthews coefficient (V M) of 2.90 Å3 Da-1; the solvent content is estimated to be 57.6%.
AB - Membrane type 1 matrix metalloproteinase (MT1-MMP) belongs to the large family of zinc-dependent endopeptidases termed MMPs that are located in the extracellular matrix. MT1-MMP was crystallized at 277 K using the vapour-diffusion method with PEG as a precipitating agent. Data sets for MT1-MMP were collected to 2.24 Å resolution at 100 K. The crystals belonged to space group P43212, with unit-cell parameters a = 62.99, c = 122.60 Å. The crystal contained one molecule per asymmetric unit, with a Matthews coefficient (V M) of 2.90 Å3 Da-1; the solvent content is estimated to be 57.6%.
UR - http://www.scopus.com/inward/record.url?scp=84905446753&partnerID=8YFLogxK
U2 - 10.1107/S2053230X13034857
DO - 10.1107/S2053230X13034857
M3 - مقالة
SN - 1744-3091
VL - 70
SP - 232
EP - 235
JO - Acta Crystallographica Section F-Structural Biology And Crystallization Communications
JF - Acta Crystallographica Section F-Structural Biology And Crystallization Communications
IS - 2
ER -