@inbook{bc6778fa26e4499d92f7f2b728362222,
title = "CryoSTEM tomography in biology",
abstract = "Electron cryo-tomography using the scanning transmission modality (STEM)enables 3D reconstruction of unstained, vitrified specimens as thick as 1 μm or more. Contrast is related to mass/thickness and atomic number, providing quantifiable chemical characterization and mass mapping of intact prokaryotic and eukaryotic cells. Energy dispersive X-ray spectroscopy by STEM provides a simple, on-the-spot chemical identification of the elemental composition in sub-cellular organic bodies or mineral deposits. This chapter provides basic background and practical information for performing cryo-STEM tomography on vitrified biological cells.",
author = "Wolf, {Sharon G.} and Michael Elbaum",
note = "We thank Dr. Lothar Houben and Prof. Peter Rez for helpful discussions, and Drs. Anna Carlsson and Maarten Wirix from Thermo Fisher Scientific for discussions and guidance on our new systems. Our work on STEM development has been supported by the Israel Science Foundation.",
year = "2019",
doi = "10.1016/bs.mcb.2019.04.001",
language = "الإنجليزيّة",
isbn = "9780128170182",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "197--215",
editor = "Thomas M{\"u}ller-Reichert and Gaia Pigino",
booktitle = "Three-Dimensional Electron Microscopy",
address = "الولايات المتّحدة",
}