CRISPR based development of RNA editing and the diagnostic platform

Lakkakula Satish; Gunamalai Lavanya; Thirupathi Kasthuri; Aruchamy Kalaivaani; Sasanala Shamili; Pandiyan Muthuramalingam; Shanmugaraj Gowrishankar; Shunmugiah Karutha Pandian; Vijai Singh; Yaron Sitrit; Ariel Kushmaro

doi:https://doi.org/10.1016/bs.pmbts.2020.12.015

CRISPR based development of RNA editing and the diagnostic platform

Lakkakula Satish, Gunamalai Lavanya, Thirupathi Kasthuri, Aruchamy Kalaivaani, Sasanala Shamili, Pandiyan Muthuramalingam, Shanmugaraj Gowrishankar, Shunmugiah Karutha Pandian, Vijai Singh, Yaron Sitrit, Ariel Kushmaro

Ben-Gurion University of the Negev

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Clustered Regularly Interspersed Short Palindromic Repeat-CRISPR-Associated (CRISPR-Cas) system has improved the ability to edit and control gene expression as desired. Genome editing approaches are currently leading the biomedical research with improved focus on direct nuclease dependent editing. So far, the research was predominantly intended on genome editing over the DNA level, recent adapted techniques are initiating to secure momentum through their proficiency to provoke modifications in RNA sequence. Integration of this system besides to lateral flow method allows reliable, quick, sensitive, precise and inexpensive diagnostic. These interesting methods illustrate only a small proportion of what is technically possible for this novel technology, but several technological obstacles need to be overcome prior to the CRISPR-Cas genome editing system can meet its full ability. This chapter covers the particulars on recent advances in CRISPR-Cas9 genome editing technology including diagnosis and technical advancements, followed by molecular mechanism of CRISPR-based RNA editing and diagnostic tools and types, and CRISPR-Cas-based biosensors.

Original language	American English
Title of host publication	Reprogramming the Genome
Subtitle of host publication	Applications of CRISPR-Cas in Non-mammalian Systems Part A
Editors	Vijai Singh
Publisher	Elsevier B.V.
Pages	117-159
Number of pages	43
ISBN (Print)	9780323853217
DOIs	https://doi.org/10.1016/bs.pmbts.2020.12.015
State	Published - 1 Jan 2021

Publication series

Name	Progress in Molecular Biology and Translational Science
Volume	179

Keywords

Biosensors
CRISPR-Cas system
Genome editing
Non-coding RNA
Targeted gene repair

All Science Journal Classification (ASJC) codes

Molecular Medicine
Molecular Biology

Access to Document

https://doi.org/10.1016/bs.pmbts.2020.12.015

Cite this

Satish, L., Lavanya, G., Kasthuri, T., Kalaivaani, A., Shamili, S., Muthuramalingam, P., Gowrishankar, S., Pandian, S. K., Singh, V., Sitrit, Y., & Kushmaro, A. (2021). CRISPR based development of RNA editing and the diagnostic platform. In V. Singh (Ed.), Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part A (pp. 117-159). (Progress in Molecular Biology and Translational Science; Vol. 179). Elsevier B.V.. https://doi.org/10.1016/bs.pmbts.2020.12.015

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abstract = "Clustered Regularly Interspersed Short Palindromic Repeat-CRISPR-Associated (CRISPR-Cas) system has improved the ability to edit and control gene expression as desired. Genome editing approaches are currently leading the biomedical research with improved focus on direct nuclease dependent editing. So far, the research was predominantly intended on genome editing over the DNA level, recent adapted techniques are initiating to secure momentum through their proficiency to provoke modifications in RNA sequence. Integration of this system besides to lateral flow method allows reliable, quick, sensitive, precise and inexpensive diagnostic. These interesting methods illustrate only a small proportion of what is technically possible for this novel technology, but several technological obstacles need to be overcome prior to the CRISPR-Cas genome editing system can meet its full ability. This chapter covers the particulars on recent advances in CRISPR-Cas9 genome editing technology including diagnosis and technical advancements, followed by molecular mechanism of CRISPR-based RNA editing and diagnostic tools and types, and CRISPR-Cas-based biosensors.",

keywords = "Biosensors, CRISPR-Cas system, Genome editing, Non-coding RNA, Targeted gene repair",

author = "Lakkakula Satish and Gunamalai Lavanya and Thirupathi Kasthuri and Aruchamy Kalaivaani and Sasanala Shamili and Pandiyan Muthuramalingam and Shanmugaraj Gowrishankar and Pandian, {Shunmugiah Karutha} and Vijai Singh and Yaron Sitrit and Ariel Kushmaro",

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Satish, L, Lavanya, G, Kasthuri, T, Kalaivaani, A, Shamili, S, Muthuramalingam, P, Gowrishankar, S, Pandian, SK, Singh, V, Sitrit, Y & Kushmaro, A 2021, CRISPR based development of RNA editing and the diagnostic platform. in V Singh (ed.), Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part A. Progress in Molecular Biology and Translational Science, vol. 179, Elsevier B.V., pp. 117-159. https://doi.org/10.1016/bs.pmbts.2020.12.015

CRISPR based development of RNA editing and the diagnostic platform. / Satish, Lakkakula; Lavanya, Gunamalai; Kasthuri, Thirupathi et al.
Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part A. ed. / Vijai Singh. Elsevier B.V., 2021. p. 117-159 (Progress in Molecular Biology and Translational Science; Vol. 179).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - CRISPR based development of RNA editing and the diagnostic platform

AU - Satish, Lakkakula

AU - Lavanya, Gunamalai

AU - Kasthuri, Thirupathi

AU - Kalaivaani, Aruchamy

AU - Shamili, Sasanala

AU - Muthuramalingam, Pandiyan

AU - Gowrishankar, Shanmugaraj

AU - Pandian, Shunmugiah Karutha

AU - Singh, Vijai

AU - Sitrit, Yaron

AU - Kushmaro, Ariel

PY - 2021/1/1

Y1 - 2021/1/1

N2 - Clustered Regularly Interspersed Short Palindromic Repeat-CRISPR-Associated (CRISPR-Cas) system has improved the ability to edit and control gene expression as desired. Genome editing approaches are currently leading the biomedical research with improved focus on direct nuclease dependent editing. So far, the research was predominantly intended on genome editing over the DNA level, recent adapted techniques are initiating to secure momentum through their proficiency to provoke modifications in RNA sequence. Integration of this system besides to lateral flow method allows reliable, quick, sensitive, precise and inexpensive diagnostic. These interesting methods illustrate only a small proportion of what is technically possible for this novel technology, but several technological obstacles need to be overcome prior to the CRISPR-Cas genome editing system can meet its full ability. This chapter covers the particulars on recent advances in CRISPR-Cas9 genome editing technology including diagnosis and technical advancements, followed by molecular mechanism of CRISPR-based RNA editing and diagnostic tools and types, and CRISPR-Cas-based biosensors.

AB - Clustered Regularly Interspersed Short Palindromic Repeat-CRISPR-Associated (CRISPR-Cas) system has improved the ability to edit and control gene expression as desired. Genome editing approaches are currently leading the biomedical research with improved focus on direct nuclease dependent editing. So far, the research was predominantly intended on genome editing over the DNA level, recent adapted techniques are initiating to secure momentum through their proficiency to provoke modifications in RNA sequence. Integration of this system besides to lateral flow method allows reliable, quick, sensitive, precise and inexpensive diagnostic. These interesting methods illustrate only a small proportion of what is technically possible for this novel technology, but several technological obstacles need to be overcome prior to the CRISPR-Cas genome editing system can meet its full ability. This chapter covers the particulars on recent advances in CRISPR-Cas9 genome editing technology including diagnosis and technical advancements, followed by molecular mechanism of CRISPR-based RNA editing and diagnostic tools and types, and CRISPR-Cas-based biosensors.

KW - Biosensors

KW - CRISPR-Cas system

KW - Genome editing

KW - Non-coding RNA

KW - Targeted gene repair

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DO - https://doi.org/10.1016/bs.pmbts.2020.12.015

M3 - Chapter

C2 - 33785175

SN - 9780323853217

T3 - Progress in Molecular Biology and Translational Science

SP - 117

EP - 159

BT - Reprogramming the Genome

A2 - Singh, Vijai

PB - Elsevier B.V.

ER -

Satish L, Lavanya G, Kasthuri T, Kalaivaani A, Shamili S, Muthuramalingam P et al. CRISPR based development of RNA editing and the diagnostic platform. In Singh V, editor, Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part A. Elsevier B.V. 2021. p. 117-159. (Progress in Molecular Biology and Translational Science). doi: https://doi.org/10.1016/bs.pmbts.2020.12.015

CRISPR based development of RNA editing and the diagnostic platform

Abstract

Publication series

Keywords

All Science Journal Classification (ASJC) codes

Access to Document

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