CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA

Meir Shamay, Nicholas Hand, M. Victor Lemas, Henry B. Koon, Susan E. Krown, John Wrangle, Prashant Desai, Juan Carlos Ramos, Richard F. Ambinder

Research output: Contribution to journalArticlepeer-review

Abstract

We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.

Original languageEnglish
Pages (from-to)1095-1099
Number of pages5
JournalJournal of Infectious Diseases
Volume205
Issue number7
DOIs
StatePublished - 1 Apr 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Immunology and Allergy

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