Coupled pre-mRNA and mRNA dynamics unveil operational strategies underlying transcriptional responses to stimuli

Amit Zeisel, Wolfgang J. Köstler, Natali Molotski, Jonathan M. Tsai, Rita Krauthgamer, Jasmine Jacob-Hirsch, Gideon Rechavi, Yoav Soen, Steffen Jung, Yosef Yarden, Eytan Domany

Research output: Contribution to journalReview articlepeer-review

Abstract

Transcriptional responses to extracellular stimuli involve tuning the rates of transcript production and degradation. Here, we show that the time-dependent profiles of these rates can be inferred from simultaneous measurements of precursor mRNA (pre-mRNA) and mature mRNA profiles. Transcriptome-wide measurements demonstrate that genes with similar mRNA profiles often exhibit marked differences in the amplitude and onset of their production rate. The latter is characterized by a large dynamic range, with a group of genes exhibiting an unexpectedly strong transient production overshoot, thereby accelerating their induction and, when combined with time-dependent degradation, shaping transient responses with precise timing and amplitude.

Original languageEnglish
Article number529
JournalMolecular Systems Biology
Volume7
DOIs
StatePublished - 13 Sep 2011

Keywords

  • half-life
  • operational strategy
  • pre-mRNA
  • transcriptional response

All Science Journal Classification (ASJC) codes

  • General Immunology and Microbiology
  • Applied Mathematics
  • General Biochemistry,Genetics and Molecular Biology
  • General Agricultural and Biological Sciences

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