TY - JOUR
T1 - Copii collar defines the boundary between er and er exit site and does not coat cargo containers
AU - Shomron, Olga
AU - Nevo-Yassaf, Inbar
AU - Aviad, Tamar
AU - Yaffe, Yakey
AU - Zahavi, Eitan Erez
AU - Dukhovny, Anna
AU - Perlson, Eran
AU - Brodsky, Ilya
AU - Yeheskel, Adva
AU - Pasmanik-Chor, Metsada
AU - Mironov, Anna
AU - Beznoussenko, Galina V.
AU - Mironov, Alexander A.
AU - Sklan, Ella H.
AU - Patterson, George H.
AU - Yonemura, Yoji
AU - Sannai, Mara
AU - Kaether, Christoph
AU - Hirschberg, Koret
N1 - Publisher Copyright: © 2021 Shomron et al.
PY - 2021
Y1 - 2021
N2 - COPII and COPI mediate the formation of membrane vesicles translocating in opposite directions within the secretory pathway. Live-cell and electron microscopy revealed a novel mode of function for COPII during cargo export from the ER. COPII is recruited to membranes defining the boundary between the ER and ER exit sites, facilitating selective cargo concentration. Using direct observation of living cells, we monitored cargo selection processes, accumulation, and fission of COPII-free ERES membranes. CRISPR/Cas12a tagging, the RUSH system, and pharmaceutical and genetic perturbations of ER-Golgi transport demonstrated that the COPII coat remains bound to the ER–ERES boundary during protein export. Manipulation of the cargobinding domain in COPII Sec24B prohibits cargo accumulation in ERES. These findings suggest a role for COPII in selecting and concentrating exported cargo rather than coating Golgi-bound carriers. These findings transform our understanding of coat proteins’ role in ER-to-Golgi transport.
AB - COPII and COPI mediate the formation of membrane vesicles translocating in opposite directions within the secretory pathway. Live-cell and electron microscopy revealed a novel mode of function for COPII during cargo export from the ER. COPII is recruited to membranes defining the boundary between the ER and ER exit sites, facilitating selective cargo concentration. Using direct observation of living cells, we monitored cargo selection processes, accumulation, and fission of COPII-free ERES membranes. CRISPR/Cas12a tagging, the RUSH system, and pharmaceutical and genetic perturbations of ER-Golgi transport demonstrated that the COPII coat remains bound to the ER–ERES boundary during protein export. Manipulation of the cargobinding domain in COPII Sec24B prohibits cargo accumulation in ERES. These findings suggest a role for COPII in selecting and concentrating exported cargo rather than coating Golgi-bound carriers. These findings transform our understanding of coat proteins’ role in ER-to-Golgi transport.
UR - http://www.scopus.com/inward/record.url?scp=85104485956&partnerID=8YFLogxK
U2 - https://doi.org/10.1083/jcb.201907224
DO - https://doi.org/10.1083/jcb.201907224
M3 - مقالة
C2 - 33852719
SN - 0021-9525
VL - 220
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 6
M1 - e201907224
ER -