TY - JOUR
T1 - Connecting the Oxidative Potential of Secondary Organic Aerosols with Reactive Oxygen Species in Exposed Lung Cells
AU - Chowdhury, Pratiti Home
AU - He, Quanfu
AU - Carmieli, Raanan
AU - Li, Chunlin
AU - Rudich, Yinon
AU - Pardo, Michal
N1 - This study was partially funded by the German Israeli Science Foundation (#I-1338-307.8/2016) and is part of the Helmholtz International Lab aeroHEALTH (www.aerohealth.eu). The work was partially supported by a research grant from the Herbert L. Janowsky Lung Cancer Research Fund, Adam Glickman, Eric Gordon, Alex Rotzang, the David M. Polen Charitable Trust, The Benoziyo Endowment Fund for the Advancement of Science, and the Midwest Electron Microscope Project. Q.H. is supported by the Koshland Foundation and the Center for Planetary Sciences, Weizmann Institute of Science. P.H.C. and Q.H. made equal contributions.
PY - 2019/12/3
Y1 - 2019/12/3
N2 - It has been hypothesized that the cytotoxicity of secondary organic aerosols (SOA) is mediated through the formation of reactive oxygen species (ROS) in the exposed cells. Here, lung epithelial cells (A549) residing at the air-liquid interface were exposed to proxies of anthropogenic and biogenic SOA that were photochemically aged under varying nitrogen oxide (NOx) concentrations in an oxidation flow reactor. The total organic peroxides and ROS radical content in the SOA were quantified by the iodometric spectrophotometric method and by continuous-wave electron paramagnetic resonance. The effect of the exposure was evaluated by measuring cell viability and cellular ROS production following the exposure. The results demonstrate that SOA that aged in the absence of NOx contained more ROS than fresh SOA and were more toxic toward the cells, while varying NOx conditions had no significant influence on levels of the ROS content in fresh SOA and their toxicity. Analysis of ROS in the exposed cells using flow cytometry showed a similar trend with the total ROS content in the SOA. This study provides a first and direct observation of such association.
AB - It has been hypothesized that the cytotoxicity of secondary organic aerosols (SOA) is mediated through the formation of reactive oxygen species (ROS) in the exposed cells. Here, lung epithelial cells (A549) residing at the air-liquid interface were exposed to proxies of anthropogenic and biogenic SOA that were photochemically aged under varying nitrogen oxide (NOx) concentrations in an oxidation flow reactor. The total organic peroxides and ROS radical content in the SOA were quantified by the iodometric spectrophotometric method and by continuous-wave electron paramagnetic resonance. The effect of the exposure was evaluated by measuring cell viability and cellular ROS production following the exposure. The results demonstrate that SOA that aged in the absence of NOx contained more ROS than fresh SOA and were more toxic toward the cells, while varying NOx conditions had no significant influence on levels of the ROS content in fresh SOA and their toxicity. Analysis of ROS in the exposed cells using flow cytometry showed a similar trend with the total ROS content in the SOA. This study provides a first and direct observation of such association.
UR - https://www.scopus.com/pages/publications/85075174375
U2 - 10.1021/acs.est.9b04449
DO - 10.1021/acs.est.9b04449
M3 - Article
C2 - 31652049
SN - 0013-936X
VL - 53
SP - 13949
EP - 13958
JO - Environmental Science and Technology
JF - Environmental Science and Technology
IS - 23
ER -