TY - JOUR
T1 - Comprehensive annotations of human herpesvirus 6A and 6B genomes reveal novel and conserved genomic features
AU - Finkel, Yaara
AU - Schmiedel, Dominik
AU - Tai-Schmiedel, Julie
AU - Nachshon, Aharon
AU - Winkler, Roni
AU - Dobesova, Martina
AU - Schwartz, Michal
AU - Mandelboim, Ofer
AU - Stern-Ginossar, Noam
N1 - Publisher Copyright: © Finkel et al.
PY - 2020/1/16
Y1 - 2020/1/16
N2 - Human herpesvirus-6 (HHV-6) A and B are ubiquitous betaherpesviruses, infecting the majority of the human population. They encompass large genomes and our understanding of their protein coding potential is far from complete. Here, we employ ribosome-profiling and systematic transcript-analysis to experimentally define HHV-6 translation products. We identify hundreds of new open reading frames (ORFs), including upstream ORFs (uORFs) and internal ORFs (iORFs), generating a complete unbiased atlas of HHV-6 proteome. By integrating systematic data from the prototypic betaherpesvirus, human cytomegalovirus, we uncover numerous uORFs and iORFs conserved across betaherpesviruses and we show uORFs are enriched in late viral genes. We identified three highly abundant HHV-6 encoded long non-coding RNAs, one of which generates a non-polyadenylated stable intron appearing to be a conserved feature of betaherpesviruses. Overall, our work reveals the complexity of HHV-6 genomes and highlights novel features conserved between betaherpesviruses, providing a rich resource for future functional studies.
AB - Human herpesvirus-6 (HHV-6) A and B are ubiquitous betaherpesviruses, infecting the majority of the human population. They encompass large genomes and our understanding of their protein coding potential is far from complete. Here, we employ ribosome-profiling and systematic transcript-analysis to experimentally define HHV-6 translation products. We identify hundreds of new open reading frames (ORFs), including upstream ORFs (uORFs) and internal ORFs (iORFs), generating a complete unbiased atlas of HHV-6 proteome. By integrating systematic data from the prototypic betaherpesvirus, human cytomegalovirus, we uncover numerous uORFs and iORFs conserved across betaherpesviruses and we show uORFs are enriched in late viral genes. We identified three highly abundant HHV-6 encoded long non-coding RNAs, one of which generates a non-polyadenylated stable intron appearing to be a conserved feature of betaherpesviruses. Overall, our work reveals the complexity of HHV-6 genomes and highlights novel features conserved between betaherpesviruses, providing a rich resource for future functional studies.
UR - http://www.scopus.com/inward/record.url?scp=85077940789&partnerID=8YFLogxK
U2 - 10.7554/eLife.50960
DO - 10.7554/eLife.50960
M3 - مقالة
C2 - 31944176
SN - 2050-084X
VL - 9
JO - eLife
JF - eLife
M1 - e50960
ER -