Compounds that select against the tetracycline-resistance efflux pump

Laura K. Stone, Michael Baym, Tami D. Lieberman, Remy Chait, Jon Clardy, Kishony Roy

Research output: Contribution to journalArticlepeer-review


We developed a competition-based screening strategy to identify compounds that invert the selective advantage of antibiotic resistance. Using our assay, we screened over 19,000 compounds for the ability to select against the TetA tetracycline-resistance efflux pump in Escherichia coli and identified two hits, β-thujaplicin and disulfiram. Treating a tetracycline-resistant population with β-thujaplicin selects for loss of the resistance gene, enabling an effective second-phase treatment with doxycycline.

Original languageEnglish
Pages (from-to)902-904
Number of pages3
JournalNature Chemical Biology
Issue number11
StatePublished - 1 Nov 2016

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


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