Common fixation-permeabilization methods cause artifactual localization of a type II transmembrane protein

Ron Benyair; Gerardo Z. Lederkremer

doi:10.1093/jmicro/dfw035

Common fixation-permeabilization methods cause artifactual localization of a type II transmembrane protein

Ron Benyair, Gerardo Z. Lederkremer

Department of Cell Research and Immunology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

We found that a localization artifact can arise from common immunofluorescence methods. Specifically, cell fixation and permeabilization can cause mislocalization of a type II membrane-bound protein, ER mannosidase I, from its native localization in vesicles to the Golgi complex. Live cell microscopy and interestingly also mild cell fixation with paraformaldehyde without membrane permeabilization do not present this artifact.

Original language	English
Pages (from-to)	517-521
Number of pages	5
Journal	Microscopy (Oxford, England)
Volume	65
Issue number	6
DOIs	https://doi.org/10.1093/jmicro/dfw035
State	Published - 1 Dec 2016

Keywords

Golgi
fixation
mannosidase
permeabilization
transmembrane protein
vesicles

All Science Journal Classification (ASJC) codes

Structural Biology
Instrumentation
Radiology Nuclear Medicine and imaging

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10.1093/jmicro/dfw035

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title = "Common fixation-permeabilization methods cause artifactual localization of a type II transmembrane protein",

abstract = "We found that a localization artifact can arise from common immunofluorescence methods. Specifically, cell fixation and permeabilization can cause mislocalization of a type II membrane-bound protein, ER mannosidase I, from its native localization in vesicles to the Golgi complex. Live cell microscopy and interestingly also mild cell fixation with paraformaldehyde without membrane permeabilization do not present this artifact.",

keywords = "Golgi, fixation, mannosidase, permeabilization, transmembrane protein, vesicles",

author = "Ron Benyair and Lederkremer, \{Gerardo Z.\}",

note = "Publisher Copyright: {\textcopyright} The Author 2016. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved.For permissions, please e-mail: [email protected].",

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AU - Lederkremer, Gerardo Z.

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PY - 2016/12/1

Y1 - 2016/12/1

N2 - We found that a localization artifact can arise from common immunofluorescence methods. Specifically, cell fixation and permeabilization can cause mislocalization of a type II membrane-bound protein, ER mannosidase I, from its native localization in vesicles to the Golgi complex. Live cell microscopy and interestingly also mild cell fixation with paraformaldehyde without membrane permeabilization do not present this artifact.

AB - We found that a localization artifact can arise from common immunofluorescence methods. Specifically, cell fixation and permeabilization can cause mislocalization of a type II membrane-bound protein, ER mannosidase I, from its native localization in vesicles to the Golgi complex. Live cell microscopy and interestingly also mild cell fixation with paraformaldehyde without membrane permeabilization do not present this artifact.

KW - Golgi

KW - fixation

KW - mannosidase

KW - permeabilization

KW - transmembrane protein

KW - vesicles

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M3 - مقالة

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SN - 2050-5698

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JF - Microscopy (Oxford, England)

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ER -

Common fixation-permeabilization methods cause artifactual localization of a type II transmembrane protein

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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