Collective conformations of DNA polymers assembled on surface density gradients

Gabriel Shemer, Yahel Atsmon, Eyal Karzbrun, Roy H. Bar-Ziv

Research output: Contribution to journalArticlepeer-review

Abstract

To study dense double-stranded DNA (dsDNA) polymer phases, we fabricated continuous density gradients of binding sites for assembly on a photochemical interface and measured both dsDNA occupancy and extension using evanescent fluorescence. Despite the abundance of available binding sites, the dsDNA density saturates after occupation of only a fraction of the available sites along the gradient. The spatial position at which the density saturates marks the onset of collective stretching of dsDNA, a direct manifestation of balancing entropic and excluded-volume interactions. The methodology presented here offers a new means to investigate dense dsDNA compartments.

Original languageAmerican English
Pages (from-to)3954-3956
Number of pages3
JournalJournal of the American Chemical Society
Volume134
Issue number9
DOIs
StatePublished - 7 Mar 2012

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Biochemistry
  • Catalysis
  • Colloid and Surface Chemistry

Fingerprint

Dive into the research topics of 'Collective conformations of DNA polymers assembled on surface density gradients'. Together they form a unique fingerprint.

Cite this