Coalescence-assisted generation of single nanoliter droplets with predefined composition

Jonathan Shemesh, Avital Nir, Avishay Bransky, Shulamit Levenberg

Research output: Contribution to journalArticlepeer-review


We demonstrate the generation of highly accurate nanoliter droplets with a predefined composition. This composition control over a single droplet is achieved by merging two droplets with known concentrations and defined volumes. A forced coalescence is accomplished by synchronizing two piezoelectric-based active droplet generators. A microscope-mounted CCD camera is used to record, quantify and monitor the process to assure its high fidelity. The device is disposable, surfactant free, simple to operate and does not require microelectrode fabrication. It delivers a single on-demand droplet with adjustable high resolution mixing ratios up to 9 at a volume range of 1-10 nanoliters. The presented platform offers, for the first time, a means to perform droplet-based high-throughput screening in the nanoliter range.

Original languageEnglish
Pages (from-to)3225-3230
Number of pages6
JournalLab on a Chip
Issue number19
StatePublished - 7 Oct 2011

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering


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